The effect of heparin on trinitrobenzene sulphonic acid-induced colitis in the rat

W. Fries, E. Pagiaro, E. Canova, P. Carraro, G. Gasparini, F. Pomerri, A. Martin, C. Carlotto, E. Mazzon, G. C. Sturniolo, G. Longo

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: Reduced blood coagulability seems to protect against inflammatory bowel disease; pilot studies using heparin in patients with inflammatory bowel disease have reported positive results. Aim: To evaluate the effects of heparin treatment on microangiographic and on inflammatory parameters in experimental colitis, induced by trinitrobenzene sulphonic acid (TNBS)-ethanol. Methods: Four groups of rats: (i) controls (saline enema), TNBS-induced colitis with (ii) sham treatment (saline, s.c.), (iii) dexamethasone (0.25 mg/kg/day s.c.) and (iv) heparin (500 U/kg t.d.s., s.c.). Microangiography was performed 2 and 4 days after colitis induction. Partial thromboplastin time, colonic wet weight, macroscopic damage score and mucosal myeloperoxidase (MPO) activity were determined at day 4. Results: TNBS-induced colitis caused a reduction in visible bowel wall vessels, which was prevented by heparin (P <0.05) but not by steroids. The macroscopic damage scores and colon wet weights were similar in all colitis groups. Compared to untreated colitis the MPO activity in heparin-treated animals was of borderline significance. Conclusions: Heparin treatment improved microangiographic features and reduced inflammation to a certain degree. Steroids delayed development of colon hypoperfusion, but were ineffective on MPO activity. It remains to be determined if the observed effects are due to the antithrombotic activity of heparin or to an antiinflammatory action.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalAlimentary Pharmacology and Therapeutics
Volume12
Issue number3
DOIs
Publication statusPublished - 1998

Fingerprint

Trinitrobenzenes
Sulfonic Acids
Colitis
Heparin
Peroxidase
Inflammatory Bowel Diseases
Colon
Steroids
Weights and Measures
Partial Thromboplastin Time
Enema
Dexamethasone
Anti-Inflammatory Agents
Ethanol
Placebos
Inflammation
Therapeutics

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Fries, W., Pagiaro, E., Canova, E., Carraro, P., Gasparini, G., Pomerri, F., ... Longo, G. (1998). The effect of heparin on trinitrobenzene sulphonic acid-induced colitis in the rat. Alimentary Pharmacology and Therapeutics, 12(3), 229-236. https://doi.org/10.1046/j.1365-2036.1998.00293.x

The effect of heparin on trinitrobenzene sulphonic acid-induced colitis in the rat. / Fries, W.; Pagiaro, E.; Canova, E.; Carraro, P.; Gasparini, G.; Pomerri, F.; Martin, A.; Carlotto, C.; Mazzon, E.; Sturniolo, G. C.; Longo, G.

In: Alimentary Pharmacology and Therapeutics, Vol. 12, No. 3, 1998, p. 229-236.

Research output: Contribution to journalArticle

Fries, W, Pagiaro, E, Canova, E, Carraro, P, Gasparini, G, Pomerri, F, Martin, A, Carlotto, C, Mazzon, E, Sturniolo, GC & Longo, G 1998, 'The effect of heparin on trinitrobenzene sulphonic acid-induced colitis in the rat', Alimentary Pharmacology and Therapeutics, vol. 12, no. 3, pp. 229-236. https://doi.org/10.1046/j.1365-2036.1998.00293.x
Fries, W. ; Pagiaro, E. ; Canova, E. ; Carraro, P. ; Gasparini, G. ; Pomerri, F. ; Martin, A. ; Carlotto, C. ; Mazzon, E. ; Sturniolo, G. C. ; Longo, G. / The effect of heparin on trinitrobenzene sulphonic acid-induced colitis in the rat. In: Alimentary Pharmacology and Therapeutics. 1998 ; Vol. 12, No. 3. pp. 229-236.
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AU - Canova, E.

AU - Carraro, P.

AU - Gasparini, G.

AU - Pomerri, F.

AU - Martin, A.

AU - Carlotto, C.

AU - Mazzon, E.

AU - Sturniolo, G. C.

AU - Longo, G.

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