The effect of humoral immunity against adducted benzo[a]pyrene on DNA damage elicited by acute carcinogen exposure in Swiss mice

R. Galati, R. Crebelli, A. Zijno, L. Conti, G. Falasca, A. Verdina

Research output: Contribution to journalArticle

Abstract

Immunoglobulins G (igG) specific for benzo[a] pyrene-DNA adducts were elicited in Swiss mice by repeated subcutaneous injections of a high molecular weight benzo[a] pyrene-DNA conjugate-adjuvant mix. The immunization procedure resulted in the production of specific antibodies against adducted benzo[a]pyrene B[a]P in all treated animals. One week after completion of the immunization procedure, groups of ten immunized and ten non immunized female mice were treated by single intraperitoneal injection with two different doses of B[a]P. The mice were sacrificed 48 hours after treatment, and both liver and bone marrow cells were isolated for subsequent determinations of DNA binding and micronucleus induction, respectively. Covalent benzo[a]pyrene adducts in liver DNA were detected by competitive ELISA and the incidence of micronucleated polychromatic erythrocytes was evaluated by scoring one thousand cells per animal. The determination of DNA adducts in liver revealed significantly (p

Original languageEnglish
Pages (from-to)747-751
Number of pages5
JournalIn Vivo
Volume14
Issue number6
Publication statusPublished - 2000

Keywords

  • DNA adducts
  • ELISA
  • Host factor
  • Specific immunity

ASJC Scopus subject areas

  • Medicine(all)

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