The effect of interleukin-12 in ex-vivo expansion of human haemopoietic progenitors

F. Bertolini, D. Soligo, L. Lazzari, C. Corsini, F. Servida, G. Sirchia

Research output: Contribution to journalArticle

Abstract

We evaluated progenitor cell proliferation in cultures supplemented by different cytokine combinations in the presence or absence of IL-12. In cultures of low density cells, cytokine combinations including IL-12 were associated to a greater proliferation (up to 6.7 ± 2.5 CFU-GM fold expansion). However, in cultures of purified CD34+ cells the more efficient cytokine combination (147 ± 49 CFU-GM fold expansion) was SCF, IL-3, IL-11 and MIP-1α, and the addition of IL-12 did not further enhance expansion of progenitors. These results indicate that accessory cells, lost in CD34+ cell purification, could be in part responsible for IL-12 effect on progenitor cell proliferation. In CD34+ cell cultures the addition of IL-12 led to CD19 mRNA generation, suggesting that IL-12 acts on haemopoietic cells with both myeloid and lymphoid potential.

Original languageEnglish
Pages (from-to)935-938
Number of pages4
JournalBritish Journal of Haematology
Volume90
Issue number4
Publication statusPublished - 1995

Keywords

  • cytokines
  • expansion
  • haemopoiesis
  • haemopoietic progenitors
  • interleukin-12

ASJC Scopus subject areas

  • Hematology

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