The effect of prolonged whole-body cryostimulation treatment with different amounts of sessions on chosen pro-and anti-inflammatory cytokines levels in healthy men

Anna Lubkowska, Zbigniew Szyguła, Dariusz Chlubek, Giuseppe Banfi

Research output: Contribution to journalArticle


Cryotherapy is used in the early treatment of acute injuries (sprains, strains, fractures) yet only a few papers discuss the possible influence of whole-body cryostimulation on inflammation mechanisms or immunology. It is postulated that cold exposure can have an immunostimulating effect related to enhanced noradrenaline response and can be connected with paracrine effects. The aim of this study was to examine the effect of different sequences of whole-body cryostimulations on the level of pro-and anti-inflammatory cytokines in healthy individuals. The research involved 45 healthy men divided into three groups. The groups were subjected to 5, 10 or 20, 3-minute long whole-body cryostimulations each day at-130°C. Blood was collected for analysis before the stimulations, after completion of the whole series, and 2 weeks after completion of the series, for the examination of any long-term effect. The analysis of results showed that in response to cryostimulation, the level of ani-inflammatory cytokines IL-6 and IL-10 increased while Il-1α cytokine level decreased. It seems that the most advantageous sequence was the series of 20 cryostimulations due to the longest lasting effects of stimulation after the completion of the whole series of treatments.

Original languageEnglish
Pages (from-to)419-425
Number of pages7
JournalScandinavian Journal of Clinical and Laboratory Investigation
Issue number5
Publication statusPublished - Sep 2011



  • Anti-inflammatory cytokine
  • Proinflammatory cytokine
  • Whole-body cryostimulation

ASJC Scopus subject areas

  • Clinical Biochemistry

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