The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces

Nora Bloise, Loredana Petecchia, Gabriele Ceccarelli, Lorenzo Fassina, Cesare Usai, Federico Bertoglio, Martina Balli, Massimo Vassalli, Maria Gabriella Cusella De Angelis, Paola Gavazzo, Marcello Imbriani, Livia Visai

Research output: Contribution to journalArticle

Abstract

Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering the best strategy to promote stem cells osteogenic differentiation. In previous studies, hBM-MSCs exposed to physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces (TiO2) were shown to improve their differentiation to osteoblasts in osteogenic condition. In the present study, the effect of a daily PEMF-exposure on osteogenic differentiation of hBM-MSCs seeded onto nanostructured TiO2 (with clusters under 100 nm of dimension) was investigated. TiO2-seeded cells were exposed to PEMF (magnetic field intensity: 2 mT; intensity of induced electric field: 5 mV; frequency: 75 Hz) and examined in terms of cell physiology modifications and osteogenic differentiation. Results showed that PEMF exposure affected TiO2-seeded cells osteogenesis by interfering with selective calcium-related osteogenic pathways, and greatly enhanced hBM-MSCs osteogenic features such as the expression of early/late osteogenic genes and protein production (e.g., ALP, COL-I, osteocalcin and osteopontin) and ALP activity. Finally, PEMF-treated cells resulted to secrete into conditioned media higher amounts of BMP-2, DCN and COL-I than untreated cell cultures. These findings confirm once more the osteoinductive potential of PEMF, suggesting that its combination with TiO2 nanostructured surface might be a great option in bone tissue engineering applications.

Original languageEnglish
Article numbere0199046
JournalPLoS One
Volume13
Issue number6
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Electromagnetic Fields
Stem cells
Mesenchymal Stromal Cells
Electromagnetic fields
stem cells
Bone
bone marrow
Bone Marrow
bones
osteopontin
cell physiology
Cell Physiological Phenomena
tissue engineering
osteocalcin
Osteopontin
Bone Regeneration
titanium
osteoblasts
Osteocalcin
Osteoblasts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces. / Bloise, Nora; Petecchia, Loredana; Ceccarelli, Gabriele; Fassina, Lorenzo; Usai, Cesare; Bertoglio, Federico; Balli, Martina; Vassalli, Massimo; De Angelis, Maria Gabriella Cusella; Gavazzo, Paola; Imbriani, Marcello; Visai, Livia.

In: PLoS One, Vol. 13, No. 6, e0199046, 01.01.2018.

Research output: Contribution to journalArticle

Bloise, N, Petecchia, L, Ceccarelli, G, Fassina, L, Usai, C, Bertoglio, F, Balli, M, Vassalli, M, De Angelis, MGC, Gavazzo, P, Imbriani, M & Visai, L 2018, 'The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces', PLoS One, vol. 13, no. 6, e0199046. https://doi.org/10.1371/journal.pone.0199046
Bloise, Nora ; Petecchia, Loredana ; Ceccarelli, Gabriele ; Fassina, Lorenzo ; Usai, Cesare ; Bertoglio, Federico ; Balli, Martina ; Vassalli, Massimo ; De Angelis, Maria Gabriella Cusella ; Gavazzo, Paola ; Imbriani, Marcello ; Visai, Livia. / The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces. In: PLoS One. 2018 ; Vol. 13, No. 6.
@article{871587dad8d44707a021d551da073c73,
title = "The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces",
abstract = "Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering the best strategy to promote stem cells osteogenic differentiation. In previous studies, hBM-MSCs exposed to physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces (TiO2) were shown to improve their differentiation to osteoblasts in osteogenic condition. In the present study, the effect of a daily PEMF-exposure on osteogenic differentiation of hBM-MSCs seeded onto nanostructured TiO2 (with clusters under 100 nm of dimension) was investigated. TiO2-seeded cells were exposed to PEMF (magnetic field intensity: 2 mT; intensity of induced electric field: 5 mV; frequency: 75 Hz) and examined in terms of cell physiology modifications and osteogenic differentiation. Results showed that PEMF exposure affected TiO2-seeded cells osteogenesis by interfering with selective calcium-related osteogenic pathways, and greatly enhanced hBM-MSCs osteogenic features such as the expression of early/late osteogenic genes and protein production (e.g., ALP, COL-I, osteocalcin and osteopontin) and ALP activity. Finally, PEMF-treated cells resulted to secrete into conditioned media higher amounts of BMP-2, DCN and COL-I than untreated cell cultures. These findings confirm once more the osteoinductive potential of PEMF, suggesting that its combination with TiO2 nanostructured surface might be a great option in bone tissue engineering applications.",
author = "Nora Bloise and Loredana Petecchia and Gabriele Ceccarelli and Lorenzo Fassina and Cesare Usai and Federico Bertoglio and Martina Balli and Massimo Vassalli and {De Angelis}, {Maria Gabriella Cusella} and Paola Gavazzo and Marcello Imbriani and Livia Visai",
year = "2018",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0199046",
language = "English",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces

AU - Bloise, Nora

AU - Petecchia, Loredana

AU - Ceccarelli, Gabriele

AU - Fassina, Lorenzo

AU - Usai, Cesare

AU - Bertoglio, Federico

AU - Balli, Martina

AU - Vassalli, Massimo

AU - De Angelis, Maria Gabriella Cusella

AU - Gavazzo, Paola

AU - Imbriani, Marcello

AU - Visai, Livia

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering the best strategy to promote stem cells osteogenic differentiation. In previous studies, hBM-MSCs exposed to physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces (TiO2) were shown to improve their differentiation to osteoblasts in osteogenic condition. In the present study, the effect of a daily PEMF-exposure on osteogenic differentiation of hBM-MSCs seeded onto nanostructured TiO2 (with clusters under 100 nm of dimension) was investigated. TiO2-seeded cells were exposed to PEMF (magnetic field intensity: 2 mT; intensity of induced electric field: 5 mV; frequency: 75 Hz) and examined in terms of cell physiology modifications and osteogenic differentiation. Results showed that PEMF exposure affected TiO2-seeded cells osteogenesis by interfering with selective calcium-related osteogenic pathways, and greatly enhanced hBM-MSCs osteogenic features such as the expression of early/late osteogenic genes and protein production (e.g., ALP, COL-I, osteocalcin and osteopontin) and ALP activity. Finally, PEMF-treated cells resulted to secrete into conditioned media higher amounts of BMP-2, DCN and COL-I than untreated cell cultures. These findings confirm once more the osteoinductive potential of PEMF, suggesting that its combination with TiO2 nanostructured surface might be a great option in bone tissue engineering applications.

AB - Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering the best strategy to promote stem cells osteogenic differentiation. In previous studies, hBM-MSCs exposed to physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces (TiO2) were shown to improve their differentiation to osteoblasts in osteogenic condition. In the present study, the effect of a daily PEMF-exposure on osteogenic differentiation of hBM-MSCs seeded onto nanostructured TiO2 (with clusters under 100 nm of dimension) was investigated. TiO2-seeded cells were exposed to PEMF (magnetic field intensity: 2 mT; intensity of induced electric field: 5 mV; frequency: 75 Hz) and examined in terms of cell physiology modifications and osteogenic differentiation. Results showed that PEMF exposure affected TiO2-seeded cells osteogenesis by interfering with selective calcium-related osteogenic pathways, and greatly enhanced hBM-MSCs osteogenic features such as the expression of early/late osteogenic genes and protein production (e.g., ALP, COL-I, osteocalcin and osteopontin) and ALP activity. Finally, PEMF-treated cells resulted to secrete into conditioned media higher amounts of BMP-2, DCN and COL-I than untreated cell cultures. These findings confirm once more the osteoinductive potential of PEMF, suggesting that its combination with TiO2 nanostructured surface might be a great option in bone tissue engineering applications.

UR - http://www.scopus.com/inward/record.url?scp=85049053585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049053585&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0199046

DO - 10.1371/journal.pone.0199046

M3 - Article

AN - SCOPUS:85049053585

VL - 13

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e0199046

ER -