The effect of S-adenosylmethionine on CNS gene expression studied by cDNA microarray analysis

Rosaria A. Cavallaro, Andrea Fuso, Fabrizio D'Anselmi, Laura Seminara, Sigfrido Scarpa

Research output: Contribution to journalArticlepeer-review

Abstract

High homocysteine (Hcy) together with low S-adenosylmethionine (SAM) levels are often observed in Alzheimer disease (AD), and this could be a sign of alteration of SAM/Hcy metabolism. It has already been shown that DNA methylation is involved in amyloid-β-protein precursor (AβPP) processing and amyloid-β(Aβ) production through the regulation of Presenilin 1 (PS1) expression and that exogenous SAM can silence the gene reducing Aβ. To investigate whether SAM administration globally influenced gene expression in the brain, we analysed 588 genes of the central nervous system in SK-N-BE neuroblastoma cells, with cDNA probes derived from untreated (DM; Differentiation Medium) or SAM treated (DM + SAM) cultures. In these conditions only seven genes were modulated by SAM treatment (and therefore by DNA methylation); three were up-regulated and four down-regulated, showing low levels of modulation.

Original languageEnglish
Pages (from-to)415-419
Number of pages5
JournalJournal of Alzheimer's Disease
Volume9
Issue number4
Publication statusPublished - 2006

Keywords

  • Alzheimer's disease
  • DNA methylation
  • Gene expression
  • Homocysteine
  • S-adenosylmethionine

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology

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