The effect of S-adenosylmethionine on CNS gene expression studied by cDNA microarray analysis

Rosaria A. Cavallaro; Andrea Fuso; Fabrizio D'Anselmi; Laura Seminara; Sigfrido Scarpa

The effect of S-adenosylmethionine on CNS gene expression studied by cDNA microarray analysis

Rosaria A. Cavallaro, Andrea Fuso, Fabrizio D'Anselmi, Laura Seminara, Sigfrido Scarpa

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

High homocysteine (Hcy) together with low S-adenosylmethionine (SAM) levels are often observed in Alzheimer disease (AD), and this could be a sign of alteration of SAM/Hcy metabolism. It has already been shown that DNA methylation is involved in amyloid-β-protein precursor (AβPP) processing and amyloid-β(Aβ) production through the regulation of Presenilin 1 (PS1) expression and that exogenous SAM can silence the gene reducing Aβ. To investigate whether SAM administration globally influenced gene expression in the brain, we analysed 588 genes of the central nervous system in SK-N-BE neuroblastoma cells, with cDNA probes derived from untreated (DM; Differentiation Medium) or SAM treated (DM + SAM) cultures. In these conditions only seven genes were modulated by SAM treatment (and therefore by DNA methylation); three were up-regulated and four down-regulated, showing low levels of modulation.

Original language	English
Pages (from-to)	415-419
Number of pages	5
Journal	Journal of Alzheimer's Disease
Volume	9
Issue number	4
Publication status	Published - 2006

Keywords

Alzheimer's disease
DNA methylation
Gene expression
Homocysteine
S-adenosylmethionine

ASJC Scopus subject areas

Neuropsychology and Physiological Psychology

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title = "The effect of S-adenosylmethionine on CNS gene expression studied by cDNA microarray analysis",

abstract = "High homocysteine (Hcy) together with low S-adenosylmethionine (SAM) levels are often observed in Alzheimer disease (AD), and this could be a sign of alteration of SAM/Hcy metabolism. It has already been shown that DNA methylation is involved in amyloid-β-protein precursor (AβPP) processing and amyloid-β(Aβ) production through the regulation of Presenilin 1 (PS1) expression and that exogenous SAM can silence the gene reducing Aβ. To investigate whether SAM administration globally influenced gene expression in the brain, we analysed 588 genes of the central nervous system in SK-N-BE neuroblastoma cells, with cDNA probes derived from untreated (DM; Differentiation Medium) or SAM treated (DM + SAM) cultures. In these conditions only seven genes were modulated by SAM treatment (and therefore by DNA methylation); three were up-regulated and four down-regulated, showing low levels of modulation.",

keywords = "Alzheimer's disease, DNA methylation, Gene expression, Homocysteine, S-adenosylmethionine",

author = "Cavallaro, {Rosaria A.} and Andrea Fuso and Fabrizio D'Anselmi and Laura Seminara and Sigfrido Scarpa",

year = "2006",

language = "English",

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journal = "Journal of Alzheimer's Disease",

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T1 - The effect of S-adenosylmethionine on CNS gene expression studied by cDNA microarray analysis

AU - Cavallaro, Rosaria A.

AU - Fuso, Andrea

AU - D'Anselmi, Fabrizio

AU - Seminara, Laura

AU - Scarpa, Sigfrido

PY - 2006

Y1 - 2006

N2 - High homocysteine (Hcy) together with low S-adenosylmethionine (SAM) levels are often observed in Alzheimer disease (AD), and this could be a sign of alteration of SAM/Hcy metabolism. It has already been shown that DNA methylation is involved in amyloid-β-protein precursor (AβPP) processing and amyloid-β(Aβ) production through the regulation of Presenilin 1 (PS1) expression and that exogenous SAM can silence the gene reducing Aβ. To investigate whether SAM administration globally influenced gene expression in the brain, we analysed 588 genes of the central nervous system in SK-N-BE neuroblastoma cells, with cDNA probes derived from untreated (DM; Differentiation Medium) or SAM treated (DM + SAM) cultures. In these conditions only seven genes were modulated by SAM treatment (and therefore by DNA methylation); three were up-regulated and four down-regulated, showing low levels of modulation.

AB - High homocysteine (Hcy) together with low S-adenosylmethionine (SAM) levels are often observed in Alzheimer disease (AD), and this could be a sign of alteration of SAM/Hcy metabolism. It has already been shown that DNA methylation is involved in amyloid-β-protein precursor (AβPP) processing and amyloid-β(Aβ) production through the regulation of Presenilin 1 (PS1) expression and that exogenous SAM can silence the gene reducing Aβ. To investigate whether SAM administration globally influenced gene expression in the brain, we analysed 588 genes of the central nervous system in SK-N-BE neuroblastoma cells, with cDNA probes derived from untreated (DM; Differentiation Medium) or SAM treated (DM + SAM) cultures. In these conditions only seven genes were modulated by SAM treatment (and therefore by DNA methylation); three were up-regulated and four down-regulated, showing low levels of modulation.

KW - Alzheimer's disease

KW - DNA methylation

KW - Gene expression

KW - Homocysteine

KW - S-adenosylmethionine

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