The effect of silk–gelatin bioink and TGF-β3 on mesenchymal stromal cells in 3D bioprinted chondrogenic constructs: A proteomic study

Shikha Chawla, Giovanna Desando, Elena Gabusi, Aarushi Sharma, Diego Trucco, Juhi Chakraborty, Cristina Manferdini, Mauro Petretta, Gina Lisignoli, Sourabh Ghosh

Research output: Contribution to journalArticlepeer-review

Abstract

Major limitation of 3D bioprinting is the poor understanding of the role of bioink in modulating molecular signaling pathways. Phenotypically stable engineered articular cartilage was fabricated using silk fibroin–gelatin (SF-G) bioink and progenitor cells or mature articular chondrocytes. In the current study, role of SF-G bioink in modulating in vitro chondrogenic signaling pathways in human bone marrow-derived stromal cells (hMSCs) is elucidated. The interaction between SF-G bioink and hMSCs augmented several chondrogenic pathways, including Wnt, HIF-1, and Notch. We explored the debatable role of TGF-β signaling, by assessing the differential protein expression by hMSCs-laden bioprinted constructs in the presence and absence of TGF-β3. hMSCs-laden bioprinted constructs contained a large percentage of collagen type II and Filamin-B, typical to the native articular cartilage. Hypertrophy markers were not identified following TGF-β3 addition. This is first detailed proteomics analysis to identify articular cartilage-specific pathways in SF-G-based 3D bioprinted construct. Graphic abstract: [Figure not available: see fulltext.]

Original languageEnglish
JournalJournal of Materials Research
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • 3D printing
  • Biological
  • Biomaterial
  • Degradation

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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