Abstract
Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.
| Original language | English |
|---|---|
| Pages (from-to) | 237-239 |
| Number of pages | 3 |
| Journal | Archives of Oral Biology |
| Volume | 37 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1992 |
Keywords
- dental caries
- liquid nitrogen
- oral yeasts
- saliva
ASJC Scopus subject areas
- Dentistry(all)
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Brambilla, E., Strohmenger, L., & Vogel, G. (1992). The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva. Archives of Oral Biology, 37(3), 237-239. https://doi.org/10.1016/0003-9969(92)90094-O