The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva

E. Brambilla, L. Strohmenger, G. Vogel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.

Original languageEnglish
Pages (from-to)237-239
Number of pages3
JournalArchives of Oral Biology
Volume37
Issue number3
DOIs
Publication statusPublished - 1992

Fingerprint

Saliva
Nitrogen
Yeasts
Candida albicans
Glycerol

Keywords

  • dental caries
  • liquid nitrogen
  • oral yeasts
  • saliva

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva. / Brambilla, E.; Strohmenger, L.; Vogel, G.

In: Archives of Oral Biology, Vol. 37, No. 3, 1992, p. 237-239.

Research output: Contribution to journalArticle

@article{7c7e080038a04b4f83146128e00bc836,
title = "The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva",
abstract = "Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1{\%}) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.",
keywords = "dental caries, liquid nitrogen, oral yeasts, saliva",
author = "E. Brambilla and L. Strohmenger and G. Vogel",
year = "1992",
doi = "10.1016/0003-9969(92)90094-O",
language = "English",
volume = "37",
pages = "237--239",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "PERGAMON-ELSEVIER SCIENCE LTD",
number = "3",

}

TY - JOUR

T1 - The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva

AU - Brambilla, E.

AU - Strohmenger, L.

AU - Vogel, G.

PY - 1992

Y1 - 1992

N2 - Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.

AB - Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.

KW - dental caries

KW - liquid nitrogen

KW - oral yeasts

KW - saliva

UR - http://www.scopus.com/inward/record.url?scp=0026829615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026829615&partnerID=8YFLogxK

U2 - 10.1016/0003-9969(92)90094-O

DO - 10.1016/0003-9969(92)90094-O

M3 - Article

VL - 37

SP - 237

EP - 239

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 3

ER -