Abstract
Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.
| Original language | English |
|---|---|
| Pages (from-to) | 237-239 |
| Number of pages | 3 |
| Journal | Archives of Oral Biology |
| Volume | 37 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1992 |
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Keywords
- dental caries
- liquid nitrogen
- oral yeasts
- saliva
ASJC Scopus subject areas
- Dentistry(all)
Cite this
The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva. / Brambilla, E.; Strohmenger, L.; Vogel, G.
In: Archives of Oral Biology, Vol. 37, No. 3, 1992, p. 237-239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva
AU - Brambilla, E.
AU - Strohmenger, L.
AU - Vogel, G.
PY - 1992
Y1 - 1992
N2 - Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.
AB - Stimulated whole saliva samples were collected from a group of 127 6-yr-old schoolchildren. Each sample was divided into three parts. The first two, one of which contained added glycerol, were immediately frozen in liquid nitrogen and stored at -196 °C for 2 months. The third part was transferred to the laboratory and plated on selective medium for yeasts. Colony counts of the frozen and non-frozen samples were then compared. Statistical analysis showed a highly significant correlation between the counts for frozen and unfrozen samples. A high (40.1%) prevalence of yeast carriers was found, and Candida albicans was the most frequently recovered yeast.
KW - dental caries
KW - liquid nitrogen
KW - oral yeasts
KW - saliva
UR - http://www.scopus.com/inward/record.url?scp=0026829615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026829615&partnerID=8YFLogxK
U2 - 10.1016/0003-9969(92)90094-O
DO - 10.1016/0003-9969(92)90094-O
M3 - Article
C2 - 1586284
AN - SCOPUS:0026829615
VL - 37
SP - 237
EP - 239
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 3
ER -