The effect of synthetic malaria pigment (β-haematin) on adhesion molecule expression and interleukin-6 production by human endothelial cells

Donatella Taramelli, Nicoletta Basilico, Anna Maria De Palma, Marina Saresella, Pasquale Ferrante, Luciana Mussoni, Piero Olliaro

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of synthetic malaria pigment (β-haematin, BH) on the expression of the intercellular adhesion molecule 1 (ICAM-1) and platelet endothelial cell adhesion molecule 1 (PECAM-1) and the production of interleukin-6 (IL-6) by human microvascular endothelial cells were measured using flow cytometry analysis and immunoenzymatic assay. BH alone did not affect basal levels of ICAM-1, PECAM-1 or IL-G. When added to cell cultures before or with, but not after, lipopolysaccharide or tumour necrosis factor α, BH at 1-100 μg/mL induced a dose-dependent inhibition of ICAM-1 and PECAM-1 expression and IL-6 production. Cell viability and human leucocyte antigen A, B, C expression remained unaffected. Similar, though more variable, results were obtained using human umbilical vein endothelial cells. These results suggested that accumulation of pigment within endothelial cells following repeated malaria infection reduces local inflammation and parasite sequestration through inhibition of either cytokine production or parasitized erythrocyte receptors on endothelial cells.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume92
Issue number1
DOIs
Publication statusPublished - 1998

Keywords

  • β-haematin
  • Cell adhesion molecule 1
  • Haemozoin
  • Intercellular adhesion molecule 1
  • Interleukin-6
  • Plasmodium falciparum
  • Platelet endothelial

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)

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