TY - JOUR
T1 - The effect of synthetic malaria pigment (β-haematin) on adhesion molecule expression and interleukin-6 production by human endothelial cells
AU - Taramelli, Donatella
AU - Basilico, Nicoletta
AU - De Palma, Anna Maria
AU - Saresella, Marina
AU - Ferrante, Pasquale
AU - Mussoni, Luciana
AU - Olliaro, Piero
PY - 1998
Y1 - 1998
N2 - The effects of synthetic malaria pigment (β-haematin, BH) on the expression of the intercellular adhesion molecule 1 (ICAM-1) and platelet endothelial cell adhesion molecule 1 (PECAM-1) and the production of interleukin-6 (IL-6) by human microvascular endothelial cells were measured using flow cytometry analysis and immunoenzymatic assay. BH alone did not affect basal levels of ICAM-1, PECAM-1 or IL-G. When added to cell cultures before or with, but not after, lipopolysaccharide or tumour necrosis factor α, BH at 1-100 μg/mL induced a dose-dependent inhibition of ICAM-1 and PECAM-1 expression and IL-6 production. Cell viability and human leucocyte antigen A, B, C expression remained unaffected. Similar, though more variable, results were obtained using human umbilical vein endothelial cells. These results suggested that accumulation of pigment within endothelial cells following repeated malaria infection reduces local inflammation and parasite sequestration through inhibition of either cytokine production or parasitized erythrocyte receptors on endothelial cells.
AB - The effects of synthetic malaria pigment (β-haematin, BH) on the expression of the intercellular adhesion molecule 1 (ICAM-1) and platelet endothelial cell adhesion molecule 1 (PECAM-1) and the production of interleukin-6 (IL-6) by human microvascular endothelial cells were measured using flow cytometry analysis and immunoenzymatic assay. BH alone did not affect basal levels of ICAM-1, PECAM-1 or IL-G. When added to cell cultures before or with, but not after, lipopolysaccharide or tumour necrosis factor α, BH at 1-100 μg/mL induced a dose-dependent inhibition of ICAM-1 and PECAM-1 expression and IL-6 production. Cell viability and human leucocyte antigen A, B, C expression remained unaffected. Similar, though more variable, results were obtained using human umbilical vein endothelial cells. These results suggested that accumulation of pigment within endothelial cells following repeated malaria infection reduces local inflammation and parasite sequestration through inhibition of either cytokine production or parasitized erythrocyte receptors on endothelial cells.
KW - β-haematin
KW - Cell adhesion molecule 1
KW - Haemozoin
KW - Intercellular adhesion molecule 1
KW - Interleukin-6
KW - Plasmodium falciparum
KW - Platelet endothelial
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U2 - 10.1016/S0035-9203(98)90954-3
DO - 10.1016/S0035-9203(98)90954-3
M3 - Article
C2 - 9692153
AN - SCOPUS:0031883692
VL - 92
SP - 57
EP - 62
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
SN - 0035-9203
IS - 1
ER -