The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells

A. Floridi, S. D'Atri, R. Menichini, M. L. Marcante, A. Nista, B. Silvestrini, A. Caputo, C. De Martino

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells has been investigated. The action of the drug on tumor cells was studied by addition of the drugs to cells harvested from Swiss male mice. The results may be summarized as follows: (1) Low concentrations of Gossypol increase the rate of oxygen consumption by uncoupling oxidative phosphorylation. High concentrations result in an inhibition of oxygen consumption with a mechanism that must be regarded as not directly related to the uncoupling activity. (2) Gossypol, at concentrations at which it exerts an uncoupling activity, stimulates mitochondrial ATPase which in turn increases the aerobic and anaerobic rates of lactate production. The decrease of glycolysis at high concentrations of Gossypol does not depend on the inhibition of enzymes of the glycolytic pathway, but must be ascribed to cell death. (3) The association of a low concentration of Gossypol with Lonidamine brings about a further inhibition of oxygen consumption. Moreover, Lonidamine abolishes the stimulation of glycolysis induced by Gossypol and lowers lactate production to values that are quite similar to those found with Lonidamine alone. (4) It may be concluded that the association of Gossypol and Lonidamine results in a very effective decrease of the energy requirements of cancer cells.

Original languageEnglish
Pages (from-to)322-335
Number of pages14
JournalExperimental and Molecular Pathology
Volume38
Issue number3
DOIs
Publication statusPublished - 1983

Fingerprint

Gossypol
Ehrlich Tumor Carcinoma
Energy Metabolism
Tumors
Cells
Oxygen Consumption
Glycolysis
Oxygen
Lactic Acid
Oxidative Phosphorylation
Cell death
lonidamine
Pharmaceutical Preparations
Adenosine Triphosphatases
Neoplasms
Cell Death
Enzymes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells. / Floridi, A.; D'Atri, S.; Menichini, R.; Marcante, M. L.; Nista, A.; Silvestrini, B.; Caputo, A.; De Martino, C.

In: Experimental and Molecular Pathology, Vol. 38, No. 3, 1983, p. 322-335.

Research output: Contribution to journalArticle

Floridi, A. ; D'Atri, S. ; Menichini, R. ; Marcante, M. L. ; Nista, A. ; Silvestrini, B. ; Caputo, A. ; De Martino, C. / The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells. In: Experimental and Molecular Pathology. 1983 ; Vol. 38, No. 3. pp. 322-335.
@article{b3887f6713e841f78c475d22ec105e35,
title = "The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells",
abstract = "The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells has been investigated. The action of the drug on tumor cells was studied by addition of the drugs to cells harvested from Swiss male mice. The results may be summarized as follows: (1) Low concentrations of Gossypol increase the rate of oxygen consumption by uncoupling oxidative phosphorylation. High concentrations result in an inhibition of oxygen consumption with a mechanism that must be regarded as not directly related to the uncoupling activity. (2) Gossypol, at concentrations at which it exerts an uncoupling activity, stimulates mitochondrial ATPase which in turn increases the aerobic and anaerobic rates of lactate production. The decrease of glycolysis at high concentrations of Gossypol does not depend on the inhibition of enzymes of the glycolytic pathway, but must be ascribed to cell death. (3) The association of a low concentration of Gossypol with Lonidamine brings about a further inhibition of oxygen consumption. Moreover, Lonidamine abolishes the stimulation of glycolysis induced by Gossypol and lowers lactate production to values that are quite similar to those found with Lonidamine alone. (4) It may be concluded that the association of Gossypol and Lonidamine results in a very effective decrease of the energy requirements of cancer cells.",
author = "A. Floridi and S. D'Atri and R. Menichini and Marcante, {M. L.} and A. Nista and B. Silvestrini and A. Caputo and {De Martino}, C.",
year = "1983",
doi = "10.1016/0014-4800(83)90072-2",
language = "English",
volume = "38",
pages = "322--335",
journal = "Experimental and Molecular Pathology",
issn = "0014-4800",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells

AU - Floridi, A.

AU - D'Atri, S.

AU - Menichini, R.

AU - Marcante, M. L.

AU - Nista, A.

AU - Silvestrini, B.

AU - Caputo, A.

AU - De Martino, C.

PY - 1983

Y1 - 1983

N2 - The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells has been investigated. The action of the drug on tumor cells was studied by addition of the drugs to cells harvested from Swiss male mice. The results may be summarized as follows: (1) Low concentrations of Gossypol increase the rate of oxygen consumption by uncoupling oxidative phosphorylation. High concentrations result in an inhibition of oxygen consumption with a mechanism that must be regarded as not directly related to the uncoupling activity. (2) Gossypol, at concentrations at which it exerts an uncoupling activity, stimulates mitochondrial ATPase which in turn increases the aerobic and anaerobic rates of lactate production. The decrease of glycolysis at high concentrations of Gossypol does not depend on the inhibition of enzymes of the glycolytic pathway, but must be ascribed to cell death. (3) The association of a low concentration of Gossypol with Lonidamine brings about a further inhibition of oxygen consumption. Moreover, Lonidamine abolishes the stimulation of glycolysis induced by Gossypol and lowers lactate production to values that are quite similar to those found with Lonidamine alone. (4) It may be concluded that the association of Gossypol and Lonidamine results in a very effective decrease of the energy requirements of cancer cells.

AB - The effect of the association of Gossypol and Lonidamine on the energy metabolism of Ehrlich ascites tumor cells has been investigated. The action of the drug on tumor cells was studied by addition of the drugs to cells harvested from Swiss male mice. The results may be summarized as follows: (1) Low concentrations of Gossypol increase the rate of oxygen consumption by uncoupling oxidative phosphorylation. High concentrations result in an inhibition of oxygen consumption with a mechanism that must be regarded as not directly related to the uncoupling activity. (2) Gossypol, at concentrations at which it exerts an uncoupling activity, stimulates mitochondrial ATPase which in turn increases the aerobic and anaerobic rates of lactate production. The decrease of glycolysis at high concentrations of Gossypol does not depend on the inhibition of enzymes of the glycolytic pathway, but must be ascribed to cell death. (3) The association of a low concentration of Gossypol with Lonidamine brings about a further inhibition of oxygen consumption. Moreover, Lonidamine abolishes the stimulation of glycolysis induced by Gossypol and lowers lactate production to values that are quite similar to those found with Lonidamine alone. (4) It may be concluded that the association of Gossypol and Lonidamine results in a very effective decrease of the energy requirements of cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=0020623419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020623419&partnerID=8YFLogxK

U2 - 10.1016/0014-4800(83)90072-2

DO - 10.1016/0014-4800(83)90072-2

M3 - Article

VL - 38

SP - 322

EP - 335

JO - Experimental and Molecular Pathology

JF - Experimental and Molecular Pathology

SN - 0014-4800

IS - 3

ER -