The effect of trehalose on human lung fibroblasts stored in euro-collins and low potassium dextran solutions

P. Carbognani, M. Rusca, L. Spaggiari, P. Solli, R. Alfieri, L. Cattelani, A. Bobbio, P. Bobbio

Research output: Contribution to journalArticle

Abstract

Background. The research on lung preservation for transplantation is directed to synthesize a solution, in order to prolong the preservation period, thus avoiding the damage to lung tissues caused by ischemia. In recent years some in vivo experimental papers have been published, in which good results were obtained by adding trehalose, a non-reducing disaccharide able to stabilize the cell membrane, to the storage solutions. Materials and methods. In this study we have evaluated the effects on human lung fibroblasts of commonly used storage solutions like Euro-Collins (EC) and Low Potassium Dextran (LPD), in which trehalose has respectively replaced glucose (T-EC) or was added (LPD-T). The cultures were incubated for 8 hours at 10°C in standard and modified solutions. Results. The data relative to EC compared to EC-T showed that the total protein content and the rate of protein synthesis, that is in other words cell viability, are drastically compromised. Concerning LPD-T, compared to standard LPD, a slightly decrease of the total protein content and of the rate of protein synthesis in the cells incubated in the modified solution were observed. The reasons for these unexpected data are related to the hypothesis that during the ischemic period membrane stabilization is neither the main nor the only factor responsible for the cellular damage. Moreover the role of trehalose in preservation solutions, is still not clear and should be further investigated. Conclusions. With the limits of the study of single cells in vitro, the advantages of trehalose containing solutions proved ineffective and toxic especially as for as EC-T is concerned.

Original languageEnglish
Pages (from-to)669-671
Number of pages3
JournalJournal of Cardiovascular Surgery
Volume38
Issue number6
Publication statusPublished - Dec 1997

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Keywords

  • Lung
  • Organ preservation methods
  • Trehalose

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Carbognani, P., Rusca, M., Spaggiari, L., Solli, P., Alfieri, R., Cattelani, L., Bobbio, A., & Bobbio, P. (1997). The effect of trehalose on human lung fibroblasts stored in euro-collins and low potassium dextran solutions. Journal of Cardiovascular Surgery, 38(6), 669-671.