The effect of vaginal candidiasis on the shedding of human immunodeficiency virus in cervicovaginal secretions

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Abstract

Objective: The purpose of this study was to evaluate the influence of symptomatic vulvovaginal candidiasis on the shedding of HIV-1 in cervicovaginal secretions of HIV-1-infected women. Study design: We obtained paired blood and cervicovaginal lavage samples from 66 HIV-infected women with symptomatic vulvovaginal candidiasis, and 249 HIV-infected control patients without genital infection. HIV-1 RNA in plasma, proviral HIV-1 DNA, HIV-1 RNA transcripts, and cell-free HIV-1 RNA in cervicovaginal secretions were quantitatively evaluated by competitive polymerase chain reaction (cPCR) and reverse transcriptase PCR (cRT-PCR). We used logistic regression on ordered data to assess the influence of vulvovaginal candidiasis on the HIV-1 load in cervicovaginal secretions adjusting for potential confounders. Results: Overall, the amount of HIV-1 RNA in plasma was significantly correlated with HIV-1 DNA (Spearman rank 0.153 ± 0.059, P =. 006), HIV-1 RNA transcripts (Spearman rank 0.169 ± 0.058, P =. 003), and cell free HIV-1 RNA (Spearman ank 0.185 ± 0.059, P =. 001) load in cervicovaginal secretion. Forty-eight out of 182 (26.4%) patients who tested negative for HIV-1 RNA in plasma were positive for HIV-DNA in their cervicovaginal secretions. In logistic regression analysis vulvovaginal candidiasis was significantly associated with increasing loads of HIV-1 RNA transcripts (Odds ratio [OR] 1.97, 95% CI 1.09-3.57, P =. 025) and cell free HIV-1 RNA (OR 2.03, 95% CI 1.10-3.73, P =. 02) in cervicovaginal secretions. Conclusion: In HIV-infected women, vulvovaginal candidiasis is associated with an increased number of copies of cell-associated and cell-free HIV-1 RNA in cervicovaginal secretions.

Original languageEnglish
Pages (from-to)774-779
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume192
Issue number3
DOIs
Publication statusPublished - Mar 2005

Fingerprint

Candidiasis
HIV-1
HIV
Vulvovaginal Candidiasis
RNA
DNA
Logistic Models
Odds Ratio
Therapeutic Irrigation
Reverse Transcriptase Polymerase Chain Reaction

Keywords

  • Cervicovaginal secretions
  • HIV infection
  • HIV shedding
  • Vulvovaginal candidiasis

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynaecology

Cite this

@article{af9b37cbd90f488a9f9e2abba11d4e40,
title = "The effect of vaginal candidiasis on the shedding of human immunodeficiency virus in cervicovaginal secretions",
abstract = "Objective: The purpose of this study was to evaluate the influence of symptomatic vulvovaginal candidiasis on the shedding of HIV-1 in cervicovaginal secretions of HIV-1-infected women. Study design: We obtained paired blood and cervicovaginal lavage samples from 66 HIV-infected women with symptomatic vulvovaginal candidiasis, and 249 HIV-infected control patients without genital infection. HIV-1 RNA in plasma, proviral HIV-1 DNA, HIV-1 RNA transcripts, and cell-free HIV-1 RNA in cervicovaginal secretions were quantitatively evaluated by competitive polymerase chain reaction (cPCR) and reverse transcriptase PCR (cRT-PCR). We used logistic regression on ordered data to assess the influence of vulvovaginal candidiasis on the HIV-1 load in cervicovaginal secretions adjusting for potential confounders. Results: Overall, the amount of HIV-1 RNA in plasma was significantly correlated with HIV-1 DNA (Spearman rank 0.153 ± 0.059, P =. 006), HIV-1 RNA transcripts (Spearman rank 0.169 ± 0.058, P =. 003), and cell free HIV-1 RNA (Spearman ank 0.185 ± 0.059, P =. 001) load in cervicovaginal secretion. Forty-eight out of 182 (26.4{\%}) patients who tested negative for HIV-1 RNA in plasma were positive for HIV-DNA in their cervicovaginal secretions. In logistic regression analysis vulvovaginal candidiasis was significantly associated with increasing loads of HIV-1 RNA transcripts (Odds ratio [OR] 1.97, 95{\%} CI 1.09-3.57, P =. 025) and cell free HIV-1 RNA (OR 2.03, 95{\%} CI 1.10-3.73, P =. 02) in cervicovaginal secretions. Conclusion: In HIV-infected women, vulvovaginal candidiasis is associated with an increased number of copies of cell-associated and cell-free HIV-1 RNA in cervicovaginal secretions.",
keywords = "Cervicovaginal secretions, HIV infection, HIV shedding, Vulvovaginal candidiasis",
author = "Arsenio Spinillo and Francesca Zara and Barbara Gardella and Eleonora Preti and Roberta Mainini and Renato Maserati",
year = "2005",
month = "3",
doi = "10.1016/j.ajog.2004.10.609",
language = "English",
volume = "192",
pages = "774--779",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "3",

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TY - JOUR

T1 - The effect of vaginal candidiasis on the shedding of human immunodeficiency virus in cervicovaginal secretions

AU - Spinillo, Arsenio

AU - Zara, Francesca

AU - Gardella, Barbara

AU - Preti, Eleonora

AU - Mainini, Roberta

AU - Maserati, Renato

PY - 2005/3

Y1 - 2005/3

N2 - Objective: The purpose of this study was to evaluate the influence of symptomatic vulvovaginal candidiasis on the shedding of HIV-1 in cervicovaginal secretions of HIV-1-infected women. Study design: We obtained paired blood and cervicovaginal lavage samples from 66 HIV-infected women with symptomatic vulvovaginal candidiasis, and 249 HIV-infected control patients without genital infection. HIV-1 RNA in plasma, proviral HIV-1 DNA, HIV-1 RNA transcripts, and cell-free HIV-1 RNA in cervicovaginal secretions were quantitatively evaluated by competitive polymerase chain reaction (cPCR) and reverse transcriptase PCR (cRT-PCR). We used logistic regression on ordered data to assess the influence of vulvovaginal candidiasis on the HIV-1 load in cervicovaginal secretions adjusting for potential confounders. Results: Overall, the amount of HIV-1 RNA in plasma was significantly correlated with HIV-1 DNA (Spearman rank 0.153 ± 0.059, P =. 006), HIV-1 RNA transcripts (Spearman rank 0.169 ± 0.058, P =. 003), and cell free HIV-1 RNA (Spearman ank 0.185 ± 0.059, P =. 001) load in cervicovaginal secretion. Forty-eight out of 182 (26.4%) patients who tested negative for HIV-1 RNA in plasma were positive for HIV-DNA in their cervicovaginal secretions. In logistic regression analysis vulvovaginal candidiasis was significantly associated with increasing loads of HIV-1 RNA transcripts (Odds ratio [OR] 1.97, 95% CI 1.09-3.57, P =. 025) and cell free HIV-1 RNA (OR 2.03, 95% CI 1.10-3.73, P =. 02) in cervicovaginal secretions. Conclusion: In HIV-infected women, vulvovaginal candidiasis is associated with an increased number of copies of cell-associated and cell-free HIV-1 RNA in cervicovaginal secretions.

AB - Objective: The purpose of this study was to evaluate the influence of symptomatic vulvovaginal candidiasis on the shedding of HIV-1 in cervicovaginal secretions of HIV-1-infected women. Study design: We obtained paired blood and cervicovaginal lavage samples from 66 HIV-infected women with symptomatic vulvovaginal candidiasis, and 249 HIV-infected control patients without genital infection. HIV-1 RNA in plasma, proviral HIV-1 DNA, HIV-1 RNA transcripts, and cell-free HIV-1 RNA in cervicovaginal secretions were quantitatively evaluated by competitive polymerase chain reaction (cPCR) and reverse transcriptase PCR (cRT-PCR). We used logistic regression on ordered data to assess the influence of vulvovaginal candidiasis on the HIV-1 load in cervicovaginal secretions adjusting for potential confounders. Results: Overall, the amount of HIV-1 RNA in plasma was significantly correlated with HIV-1 DNA (Spearman rank 0.153 ± 0.059, P =. 006), HIV-1 RNA transcripts (Spearman rank 0.169 ± 0.058, P =. 003), and cell free HIV-1 RNA (Spearman ank 0.185 ± 0.059, P =. 001) load in cervicovaginal secretion. Forty-eight out of 182 (26.4%) patients who tested negative for HIV-1 RNA in plasma were positive for HIV-DNA in their cervicovaginal secretions. In logistic regression analysis vulvovaginal candidiasis was significantly associated with increasing loads of HIV-1 RNA transcripts (Odds ratio [OR] 1.97, 95% CI 1.09-3.57, P =. 025) and cell free HIV-1 RNA (OR 2.03, 95% CI 1.10-3.73, P =. 02) in cervicovaginal secretions. Conclusion: In HIV-infected women, vulvovaginal candidiasis is associated with an increased number of copies of cell-associated and cell-free HIV-1 RNA in cervicovaginal secretions.

KW - Cervicovaginal secretions

KW - HIV infection

KW - HIV shedding

KW - Vulvovaginal candidiasis

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