The effects of α-latrotoxin of black widow spider venom on synaptosome ultrastructure. A morphometric analysis correlating its effects on transmitter release

Osamu Watanabe, Jacopo Meldolesi

Research output: Contribution to journalArticle

Abstract

The morphological effects of α-latrotoxin, the major component of black widow spider venom, were studied quantitatively in a crude synaptosome fraction (prepared from rat brain cortices) which was incubated at 37 ° C for 10 min in Ringer solutions. Two toxin concentrations were employed, one causing a very large stimulation of transmitter release (∼65% and ∼43% release of [3H]noradrenaline from preloaded synaptosomes, with and without Ca2+ in the incubation buffer), the other 50-60% as active. Incubated synaptosomes, fixed in suspension with aldehydes, were evenly dispersed in agarose before embedding, to assure randomized sampling in the subsequent morphometric analysis. In all the experimental conditions investigated, α-latrotoxin treatment caused a significant decrease in the density (number/unit area) of synaptic vesicles in synaptosome profiles. Such an effect was dose-dependent and partially Ca2+-dependent, in good agreement with the data on transmitter release. At high toxin concentration a moderate increase of synaptosome volume and surface area was observed, both with and without Ca2+. Mitochondrial swelling appeared only in synaptosomes treated in Ca2+ containing medium. These effects of α-latrotoxin are similar to those described previously at the neuromuscular junction. Thus, the toxin might be a tool of general use for studying vertebrate synapses.

Original languageEnglish
Pages (from-to)517-531
Number of pages15
JournalJournal of Neurocytology
Volume12
Issue number3
DOIs
Publication statusPublished - Jun 1983

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Synaptosomes
Mitochondrial Swelling
Synaptic Vesicles
Neuromuscular Junction
Aldehydes
Synapses
Sepharose
Vertebrates
black widow spider venom
Suspensions
Norepinephrine
Buffers
Brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Histology
  • Anatomy
  • Cell Biology

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The effects of α-latrotoxin of black widow spider venom on synaptosome ultrastructure. A morphometric analysis correlating its effects on transmitter release. / Watanabe, Osamu; Meldolesi, Jacopo.

In: Journal of Neurocytology, Vol. 12, No. 3, 06.1983, p. 517-531.

Research output: Contribution to journalArticle

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