The effects of aging on enzyme activities and metabolite concentrations in skeletal muscle from sedentary male and female subjects

Ornella Pastoris, Federica Boschi, Manuela Verri, Paola Baiardi, Giorgio Felzani, Jacopo Vecchiet, Maurizia Dossena, Mariana Catapano

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Aging affects the metabolic capacity of skeletal muscle, in particular the glycolytic and respiratory capacities. The purpose of this study was to quantify biochemical alterations due to aging in muscular metabolic capacity in human skeletal muscles in sedentary subjects. The activities of various marker enzymes and metabolites related to glycolysis, Krebs' cycle and the electron transfer chain and high energy phosphate compounds were measured in muscle biopsies from the rectus abdominis, vastus lateralis, and gluteus maximus muscles of 76 sedentary subjects (32 males and 44 females) between 15 and 91 yr. No significant differences between males and females were found, but changes related to age were: a decrease in hexokinase and lactate dehydrogenase activities in the rectus abdominis; a decrease in citrate synthase activity and citrate in the vastus lateralis; an increase in pyruvate kinase activity and a decrease in ATP and creatine phosphate concentrations in the gluteus maximus. These data suggest that distinct muscles may respond differently to aging regardless of sex in sedentary subjects. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)95-104
Number of pages10
JournalExperimental Gerontology
Volume35
Issue number1
DOIs
Publication statusPublished - Feb 2000

Fingerprint

Enzyme activity
Metabolites
Muscle
Rectus Abdominis
Skeletal Muscle
Aging of materials
Quadriceps Muscle
Muscles
Enzymes
Citrate (si)-Synthase
Hexokinase
Pyruvate Kinase
Citric Acid Cycle
Phosphocreatine
Glycolysis
L-Lactate Dehydrogenase
Citric Acid
Adenosine Triphosphate
Phosphates
Electrons

Keywords

  • Aging
  • Enzyme activity
  • Female
  • Male
  • Metabolite concentration
  • Skeletal muscle

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

The effects of aging on enzyme activities and metabolite concentrations in skeletal muscle from sedentary male and female subjects. / Pastoris, Ornella; Boschi, Federica; Verri, Manuela; Baiardi, Paola; Felzani, Giorgio; Vecchiet, Jacopo; Dossena, Maurizia; Catapano, Mariana.

In: Experimental Gerontology, Vol. 35, No. 1, 02.2000, p. 95-104.

Research output: Contribution to journalArticle

Pastoris, Ornella ; Boschi, Federica ; Verri, Manuela ; Baiardi, Paola ; Felzani, Giorgio ; Vecchiet, Jacopo ; Dossena, Maurizia ; Catapano, Mariana. / The effects of aging on enzyme activities and metabolite concentrations in skeletal muscle from sedentary male and female subjects. In: Experimental Gerontology. 2000 ; Vol. 35, No. 1. pp. 95-104.
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