The effects of exogenous sphingosine on neuro2a cells are strictly related to the overall capacity of cells to metabolize sphingosine

Laura Riboni, Alessandro Prinetti, Rosaria Bassi, Paola Viani, Guido Tettamanti

Research output: Contribution to journalArticlepeer-review

Abstract

Neuro2a cells were exposed to different doses (1-40 nmol/106 cells) of [C3-3H] sphingosine and the relationship between metabolism and biological effects of sphingosine was investigated. Sphingosine appeared to be rapidly taken up and metabolized. The incorporation of sphingosine was not merely dependent on its concentration but primarily on the dose per cell of administered sphingosine. At low doses, [3H]sphingosine represented a minor portion of the cellular radioactivity, and N-acylated metabolites, particularly ceramide, largely prevailed over degradation products. Concomitantly with ceramide increase, Neuro2a differentiation took place. With increasing exogenous sphingosine/doses, the acylation process reached saturation. From this point on, [3H]sphingosine started accumulating and eventually cell toxicity occurred. In conclusion, the biological effects exerted by exogenous sphingosine on Neuro2a cells are not merely dependent on the long-chain base concentration in the culture medium, but are strictly related to the cellular dose of exogenous sphingosine and to the capacity of cells to metabolize sphingosine.

Original languageEnglish
Pages (from-to)900-904
Number of pages5
JournalJournal of Biochemistry
Volume124
Issue number5
Publication statusPublished - Nov 1998

Keywords

  • Ceramide
  • Neuro2a cells
  • Sphingosine
  • Sphingosine effects
  • Sphingosine metabolism

ASJC Scopus subject areas

  • Biochemistry

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