Abstract
We evaluated the effectiveness of L-arginine mono(2-mercaptoethanesulfonate) (argimesna) to limit the extent of myocardial damage resulting from 60 minutes of severe ischemia followed by 30 minutes of reperfusion in the Langendorff-perfused rabbit heart. Argimesna is a sulfhydryl group containing molecule which has no effect on glutathione status or on the total thiol pool. The effects of 10 -6 M argimesna were compared with those of 10 -6 M L-arginine and of 10 -6 M sodium salt of 2-mercaptoethanesulfonate (mesna). Pretreatment of the hearts with 10 -6 M argimesna resulted in marked myocardial protection, measured in terms of improved recovery of developed pressure (p <0.01), reduced release of creatine kinase (p <0.01), maintenance of mitochondrial function and increased stores of ATP on reperfusion. On reperfusion less oxidative stress developed, as indicated by less accumulation of oxidized glutathione (p <0.01). These effects were specific for argimesna; no significant protection could be found for mesna and L-arginine. The beneficial effects of argimesna could not be explained by hemodynamic differences or effects on anaerobic metabolism. Neither is it likely that argimesna acts as a free radical scavenger at the concentrations employed. The protection may be achieved by maintenance of -SH groups during ischemia and reperfusion.
| Original language | English |
|---|---|
| Pages (from-to) | 179-187 |
| Number of pages | 9 |
| Journal | Cardioscience |
| Volume | 3 |
| Issue number | 3 |
| Publication status | Published - 1992 |
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ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
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The effects of L-arginine mono(2-mercaptoethanesulfonate) on the ischemic and reperfused heart. / Cargnoni, A.; Comini, L.; Boraso, A.; De Giuli, F.; Scotti, C.; Ferrari, R.
In: Cardioscience, Vol. 3, No. 3, 1992, p. 179-187.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of L-arginine mono(2-mercaptoethanesulfonate) on the ischemic and reperfused heart
AU - Cargnoni, A.
AU - Comini, L.
AU - Boraso, A.
AU - De Giuli, F.
AU - Scotti, C.
AU - Ferrari, R.
PY - 1992
Y1 - 1992
N2 - We evaluated the effectiveness of L-arginine mono(2-mercaptoethanesulfonate) (argimesna) to limit the extent of myocardial damage resulting from 60 minutes of severe ischemia followed by 30 minutes of reperfusion in the Langendorff-perfused rabbit heart. Argimesna is a sulfhydryl group containing molecule which has no effect on glutathione status or on the total thiol pool. The effects of 10 -6 M argimesna were compared with those of 10 -6 M L-arginine and of 10 -6 M sodium salt of 2-mercaptoethanesulfonate (mesna). Pretreatment of the hearts with 10 -6 M argimesna resulted in marked myocardial protection, measured in terms of improved recovery of developed pressure (p <0.01), reduced release of creatine kinase (p <0.01), maintenance of mitochondrial function and increased stores of ATP on reperfusion. On reperfusion less oxidative stress developed, as indicated by less accumulation of oxidized glutathione (p <0.01). These effects were specific for argimesna; no significant protection could be found for mesna and L-arginine. The beneficial effects of argimesna could not be explained by hemodynamic differences or effects on anaerobic metabolism. Neither is it likely that argimesna acts as a free radical scavenger at the concentrations employed. The protection may be achieved by maintenance of -SH groups during ischemia and reperfusion.
AB - We evaluated the effectiveness of L-arginine mono(2-mercaptoethanesulfonate) (argimesna) to limit the extent of myocardial damage resulting from 60 minutes of severe ischemia followed by 30 minutes of reperfusion in the Langendorff-perfused rabbit heart. Argimesna is a sulfhydryl group containing molecule which has no effect on glutathione status or on the total thiol pool. The effects of 10 -6 M argimesna were compared with those of 10 -6 M L-arginine and of 10 -6 M sodium salt of 2-mercaptoethanesulfonate (mesna). Pretreatment of the hearts with 10 -6 M argimesna resulted in marked myocardial protection, measured in terms of improved recovery of developed pressure (p <0.01), reduced release of creatine kinase (p <0.01), maintenance of mitochondrial function and increased stores of ATP on reperfusion. On reperfusion less oxidative stress developed, as indicated by less accumulation of oxidized glutathione (p <0.01). These effects were specific for argimesna; no significant protection could be found for mesna and L-arginine. The beneficial effects of argimesna could not be explained by hemodynamic differences or effects on anaerobic metabolism. Neither is it likely that argimesna acts as a free radical scavenger at the concentrations employed. The protection may be achieved by maintenance of -SH groups during ischemia and reperfusion.
UR - http://www.scopus.com/inward/record.url?scp=0026767411&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026767411&partnerID=8YFLogxK
M3 - Article
C2 - 1420954
AN - SCOPUS:0026767411
VL - 3
SP - 179
EP - 187
JO - Cardioscience
JF - Cardioscience
SN - 1015-5007
IS - 3
ER -