The effects of L-arginine mono(2-mercaptoethanesulfonate) on the ischemic and reperfused heart

A. Cargnoni, L. Comini, A. Boraso, F. De Giuli, C. Scotti, R. Ferrari

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We evaluated the effectiveness of L-arginine mono(2-mercaptoethanesulfonate) (argimesna) to limit the extent of myocardial damage resulting from 60 minutes of severe ischemia followed by 30 minutes of reperfusion in the Langendorff-perfused rabbit heart. Argimesna is a sulfhydryl group containing molecule which has no effect on glutathione status or on the total thiol pool. The effects of 10 -6 M argimesna were compared with those of 10 -6 M L-arginine and of 10 -6 M sodium salt of 2-mercaptoethanesulfonate (mesna). Pretreatment of the hearts with 10 -6 M argimesna resulted in marked myocardial protection, measured in terms of improved recovery of developed pressure (p <0.01), reduced release of creatine kinase (p <0.01), maintenance of mitochondrial function and increased stores of ATP on reperfusion. On reperfusion less oxidative stress developed, as indicated by less accumulation of oxidized glutathione (p <0.01). These effects were specific for argimesna; no significant protection could be found for mesna and L-arginine. The beneficial effects of argimesna could not be explained by hemodynamic differences or effects on anaerobic metabolism. Neither is it likely that argimesna acts as a free radical scavenger at the concentrations employed. The protection may be achieved by maintenance of -SH groups during ischemia and reperfusion.

Original languageEnglish
Pages (from-to)179-187
Number of pages9
JournalCardioscience
Volume3
Issue number3
Publication statusPublished - 1992

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Reperfusion
Ischemia
Maintenance
Mesna
Anaerobiosis
arginine 2-mercaptoethane sulfonate
Free Radical Scavengers
Glutathione Disulfide
Creatine Kinase
Sulfhydryl Compounds
Glutathione
Arginine
Oxidative Stress
Salts
Adenosine Triphosphate
Hemodynamics
Rabbits
Pressure

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Cargnoni, A., Comini, L., Boraso, A., De Giuli, F., Scotti, C., & Ferrari, R. (1992). The effects of L-arginine mono(2-mercaptoethanesulfonate) on the ischemic and reperfused heart. Cardioscience, 3(3), 179-187.

The effects of L-arginine mono(2-mercaptoethanesulfonate) on the ischemic and reperfused heart. / Cargnoni, A.; Comini, L.; Boraso, A.; De Giuli, F.; Scotti, C.; Ferrari, R.

In: Cardioscience, Vol. 3, No. 3, 1992, p. 179-187.

Research output: Contribution to journalArticle

Cargnoni, A, Comini, L, Boraso, A, De Giuli, F, Scotti, C & Ferrari, R 1992, 'The effects of L-arginine mono(2-mercaptoethanesulfonate) on the ischemic and reperfused heart', Cardioscience, vol. 3, no. 3, pp. 179-187.
Cargnoni, A. ; Comini, L. ; Boraso, A. ; De Giuli, F. ; Scotti, C. ; Ferrari, R. / The effects of L-arginine mono(2-mercaptoethanesulfonate) on the ischemic and reperfused heart. In: Cardioscience. 1992 ; Vol. 3, No. 3. pp. 179-187.
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