The effects of N-acetylated alpha-linked acidic dipeptidase (NAALADase) inhibitors on [3H]NAAG catabolism in vivo

Barbara L. Stauch, Michael B. Robinson, Gianluigi Forloni, Guochuan Tsai, Joseph T. Coyle

Research output: Contribution to journalArticle

Abstract

N-Acetylated, alpha-linked acidic dipeptidase (NAALADase) is a chloride-activated, membrane bound, metallopeptidase that cleaves the endogenous neuropeptide N-acetyl-aspartyl-glutamate (NAAG) in vitro. To determine whether NAALADase is the catabolic enzyme of NAAG in vivo, we have examined the effects on [3H]NAAG metabolism of intrastriatal co-injections of agents that affect NAALADase activity in vitro. Co-injections of NAALADase inhibitors, such as quisqualate (Quis), phosphate, dithiothreitol and EGTA were found to prolong the t 1 2 of [3H]NAAG, whereas cobalt, a NAALADase activity stimulator, accelerated [3H]NAAG catabolism. These results are consistent with a role for NAALADase in the extracellular disposition of endogenous NAAG.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalNeuroscience Letters
Volume100
Issue number1-3
DOIs
Publication statusPublished - May 22 1989

Keywords

  • Excitatory neurotransmission
  • Glutamate
  • n-Acetyl-aspartyl-glutamate (NAAG)
  • Peptidase
  • Quisqualate
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'The effects of N-acetylated alpha-linked acidic dipeptidase (NAALADase) inhibitors on [3H]NAAG catabolism in vivo'. Together they form a unique fingerprint.

  • Cite this