The effects of plasma electrolytically oxidized NiTi on in vitro endothelialization

Z. Huan, H. Yu, H. Li, M. S. Ruiter, J. Chang, I. Apachitei, J. Duszczyk, C. J M de Vries, L. E. Fratila-Apachitei

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The role of biomaterials surface in controlling the interfacial biological events leading to implant integration is of key importance. In this study, the effects of NiTi surfaces treated by plasma electrolytic oxidation (PEO) on human umbilical vein endothelial cells (HUVECs) have been investigated. The changes in NiTi surface morphology and chemistry were assessed by SEM, XPS and cross-section TEM/EDX analyzes whereas the effects of the resultant surfaces on in vitro endothelialization and cell junction proteins have been evaluated by life/dead staining, SEM, cells counting, qPCR and immunofluorescence. The findings indicated that the PEO-treated NiTi, with a microporous morphology and oxide dominated surface chemistry, supports viability and proliferation of HUVECs. Numerous thin filopodia probing the microporous surface assisted cells attachment. In addition, claudin-and occludin have been upregulated and expression of vascular endothelial-cadherin was not suppressed on PEO-treated NiTi relative to the reference electropolished surfaces. The results of this study suggest that novel NiTi surfaces may be developed using the PEO process, which can be of benefit to atherosclerosis treatment.

Original languageEnglish
Pages (from-to)365-373
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume141
DOIs
Publication statusPublished - May 1 2016
Externally publishedYes

Fingerprint

Plasmas
Human Umbilical Vein Endothelial Cells
Oxidation
Endothelial cells
Occludin
Surface chemistry
oxidation
Pseudopodia
Intercellular Junctions
Biocompatible Materials
veins
Oxides
Fluorescent Antibody Technique
Atherosclerosis
Scanning electron microscopy
cells
chemistry
Staining and Labeling
arteriosclerosis
Biomaterials

Keywords

  • Endothelial cells
  • Junction proteins
  • Nitinol
  • Plasma electrolytic oxidation
  • Stents

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Huan, Z., Yu, H., Li, H., Ruiter, M. S., Chang, J., Apachitei, I., ... Fratila-Apachitei, L. E. (2016). The effects of plasma electrolytically oxidized NiTi on in vitro endothelialization. Colloids and Surfaces B: Biointerfaces, 141, 365-373. https://doi.org/10.1016/j.colsurfb.2016.02.001

The effects of plasma electrolytically oxidized NiTi on in vitro endothelialization. / Huan, Z.; Yu, H.; Li, H.; Ruiter, M. S.; Chang, J.; Apachitei, I.; Duszczyk, J.; de Vries, C. J M; Fratila-Apachitei, L. E.

In: Colloids and Surfaces B: Biointerfaces, Vol. 141, 01.05.2016, p. 365-373.

Research output: Contribution to journalArticle

Huan, Z, Yu, H, Li, H, Ruiter, MS, Chang, J, Apachitei, I, Duszczyk, J, de Vries, CJM & Fratila-Apachitei, LE 2016, 'The effects of plasma electrolytically oxidized NiTi on in vitro endothelialization', Colloids and Surfaces B: Biointerfaces, vol. 141, pp. 365-373. https://doi.org/10.1016/j.colsurfb.2016.02.001
Huan, Z. ; Yu, H. ; Li, H. ; Ruiter, M. S. ; Chang, J. ; Apachitei, I. ; Duszczyk, J. ; de Vries, C. J M ; Fratila-Apachitei, L. E. / The effects of plasma electrolytically oxidized NiTi on in vitro endothelialization. In: Colloids and Surfaces B: Biointerfaces. 2016 ; Vol. 141. pp. 365-373.
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