TY - JOUR
T1 - The Eps15 homology (EH) domain-based interaction between Eps15 and Hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport
AU - Doria, Margherita
AU - Salcini, Anna Elisabetta
AU - Colombo, Emanuela
AU - Parslow, Tristram G.
AU - Pelicci, Pier Giuseppe
AU - Di Fiore, Pier Paolo
PY - 1999/12/27
Y1 - 1999/12/27
N2 - The Eps15 homology (EH) module is a protein-protein interaction domain that establishes a network of connections involved in various aspects of endocytosis and sorting. The finding that EH-containing proteins bind to Hrb (a cellular cofactor of the Rev protein) and to the related protein Hrbl raised the possibility that the EH network might also influence the so-called Rev export pathway, which mediates nucleocytoplasmic transfer of proteins and RNAs. In this study, we demonstrate that Eps15 and Eps15R, two EH-containing proteins, synergize with Hrb and Hrbl to enhance the function of Rev in the export pathway. In addition, the EH-mediated association between Eps15 and Hrb is required for the synergistic effect. The interaction between Eps15 and Hrb occurs in the cytoplasm, thus pointing to an unexpected site of action of Hrb, and to a possible role of the Eps15-Hrb complex in regulating the stability of Rev.
AB - The Eps15 homology (EH) module is a protein-protein interaction domain that establishes a network of connections involved in various aspects of endocytosis and sorting. The finding that EH-containing proteins bind to Hrb (a cellular cofactor of the Rev protein) and to the related protein Hrbl raised the possibility that the EH network might also influence the so-called Rev export pathway, which mediates nucleocytoplasmic transfer of proteins and RNAs. In this study, we demonstrate that Eps15 and Eps15R, two EH-containing proteins, synergize with Hrb and Hrbl to enhance the function of Rev in the export pathway. In addition, the EH-mediated association between Eps15 and Hrb is required for the synergistic effect. The interaction between Eps15 and Hrb occurs in the cytoplasm, thus pointing to an unexpected site of action of Hrb, and to a possible role of the Eps15-Hrb complex in regulating the stability of Rev.
KW - EH
KW - Endocytosis
KW - Eps15
KW - Hrb
KW - Nucleo-cytoplasmic transport
UR - http://www.scopus.com/inward/record.url?scp=0033611126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033611126&partnerID=8YFLogxK
U2 - 10.1083/jcb.147.7.1379
DO - 10.1083/jcb.147.7.1379
M3 - Article
C2 - 10613896
AN - SCOPUS:0033611126
VL - 147
SP - 1379
EP - 1384
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 7
ER -