The evolutionary conserved gene C16orf35 encodes a nucleo-cytoplasmic protein that interacts with p73

Andrea Lunardi, Fulvio Chiacchiera, Elisa D'Este, Marcello Carotti, Marco Dal Ferro, Giulio Di Minin, Giannino Del Sal, Licio Collavin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

C16orf35 is a highly conserved gene positioned upstream of the alpha-globins in humans and other vertebrates. The deduced protein is also highly conserved, it has no defined structural features or domains, and its function is currently unknown. Here we show that the C16orf35 protein has nuclear and cytosolic distribution, and can localize to PML nuclear bodies. The C16orf35 protein was detected in several human transformed cells lines, and studies of transient and stable overexpression indicate that increased levels of C16orf35 inhibit cell proliferation. We also find that C16orf35 interacts with human p73, and represses transcription by TAp73γ but not by TAp73α. This selectivity is not due to differential interaction, since C16orf35 binds both p73 variants. Our data suggest that C16orf35 can modulate differentially the specific activities of selected p73 isoforms.

Original languageEnglish
Pages (from-to)428-433
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume388
Issue number2
DOIs
Publication statusPublished - Oct 16 2009

Fingerprint

Genes
alpha-Globins
Cell proliferation
Transcription
Protein Isoforms
Transformed Cell Line
Proteins
Cells
Nuclear Proteins
Vertebrates
Cell Proliferation

Keywords

  • Alpha-globin regulatory element
  • C16orf35 gene
  • CG8783
  • p53-family
  • pfam03666
  • Protein-protein interaction
  • Tumor suppressor proteins
  • UPF0171

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

The evolutionary conserved gene C16orf35 encodes a nucleo-cytoplasmic protein that interacts with p73. / Lunardi, Andrea; Chiacchiera, Fulvio; D'Este, Elisa; Carotti, Marcello; Ferro, Marco Dal; Minin, Giulio Di; Sal, Giannino Del; Collavin, Licio.

In: Biochemical and Biophysical Research Communications, Vol. 388, No. 2, 16.10.2009, p. 428-433.

Research output: Contribution to journalArticle

Lunardi, Andrea ; Chiacchiera, Fulvio ; D'Este, Elisa ; Carotti, Marcello ; Ferro, Marco Dal ; Minin, Giulio Di ; Sal, Giannino Del ; Collavin, Licio. / The evolutionary conserved gene C16orf35 encodes a nucleo-cytoplasmic protein that interacts with p73. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 388, No. 2. pp. 428-433.
@article{3246931b763d4348b77802287219adea,
title = "The evolutionary conserved gene C16orf35 encodes a nucleo-cytoplasmic protein that interacts with p73",
abstract = "C16orf35 is a highly conserved gene positioned upstream of the alpha-globins in humans and other vertebrates. The deduced protein is also highly conserved, it has no defined structural features or domains, and its function is currently unknown. Here we show that the C16orf35 protein has nuclear and cytosolic distribution, and can localize to PML nuclear bodies. The C16orf35 protein was detected in several human transformed cells lines, and studies of transient and stable overexpression indicate that increased levels of C16orf35 inhibit cell proliferation. We also find that C16orf35 interacts with human p73, and represses transcription by TAp73γ but not by TAp73α. This selectivity is not due to differential interaction, since C16orf35 binds both p73 variants. Our data suggest that C16orf35 can modulate differentially the specific activities of selected p73 isoforms.",
keywords = "Alpha-globin regulatory element, C16orf35 gene, CG8783, p53-family, pfam03666, Protein-protein interaction, Tumor suppressor proteins, UPF0171",
author = "Andrea Lunardi and Fulvio Chiacchiera and Elisa D'Este and Marcello Carotti and Ferro, {Marco Dal} and Minin, {Giulio Di} and Sal, {Giannino Del} and Licio Collavin",
year = "2009",
month = "10",
day = "16",
doi = "10.1016/j.bbrc.2009.08.027",
language = "English",
volume = "388",
pages = "428--433",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The evolutionary conserved gene C16orf35 encodes a nucleo-cytoplasmic protein that interacts with p73

AU - Lunardi, Andrea

AU - Chiacchiera, Fulvio

AU - D'Este, Elisa

AU - Carotti, Marcello

AU - Ferro, Marco Dal

AU - Minin, Giulio Di

AU - Sal, Giannino Del

AU - Collavin, Licio

PY - 2009/10/16

Y1 - 2009/10/16

N2 - C16orf35 is a highly conserved gene positioned upstream of the alpha-globins in humans and other vertebrates. The deduced protein is also highly conserved, it has no defined structural features or domains, and its function is currently unknown. Here we show that the C16orf35 protein has nuclear and cytosolic distribution, and can localize to PML nuclear bodies. The C16orf35 protein was detected in several human transformed cells lines, and studies of transient and stable overexpression indicate that increased levels of C16orf35 inhibit cell proliferation. We also find that C16orf35 interacts with human p73, and represses transcription by TAp73γ but not by TAp73α. This selectivity is not due to differential interaction, since C16orf35 binds both p73 variants. Our data suggest that C16orf35 can modulate differentially the specific activities of selected p73 isoforms.

AB - C16orf35 is a highly conserved gene positioned upstream of the alpha-globins in humans and other vertebrates. The deduced protein is also highly conserved, it has no defined structural features or domains, and its function is currently unknown. Here we show that the C16orf35 protein has nuclear and cytosolic distribution, and can localize to PML nuclear bodies. The C16orf35 protein was detected in several human transformed cells lines, and studies of transient and stable overexpression indicate that increased levels of C16orf35 inhibit cell proliferation. We also find that C16orf35 interacts with human p73, and represses transcription by TAp73γ but not by TAp73α. This selectivity is not due to differential interaction, since C16orf35 binds both p73 variants. Our data suggest that C16orf35 can modulate differentially the specific activities of selected p73 isoforms.

KW - Alpha-globin regulatory element

KW - C16orf35 gene

KW - CG8783

KW - p53-family

KW - pfam03666

KW - Protein-protein interaction

KW - Tumor suppressor proteins

KW - UPF0171

UR - http://www.scopus.com/inward/record.url?scp=69249205372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249205372&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.08.027

DO - 10.1016/j.bbrc.2009.08.027

M3 - Article

C2 - 19666006

AN - SCOPUS:69249205372

VL - 388

SP - 428

EP - 433

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -