Abstract
EWS encodes a ubiquitously expressed RNA binding protein with largely unknown function. In Ewing sarcoma family tumors (EFT), one allele is rearranged with an ETS gene. This is the first description of an EFT with a complete EWS deficiency in the presence of two copies of a rearranged chromosome 22 carrying an interstitial EWS-FL11 translocation. Absence of EWS protein suggested that it is dispensable for EFT growth. By sequencing of EWS cDNA from unrelated EFTs, we excluded inactivation of EWS as a general mechanism in EFT pathogenesis. Rather, EWS was found to be uniformly expressed in two splicing variants of similar abundaney, EWSα and EWSβ, which differ in a single amino acid. Three EWS negative cell lines were established, which will serve as valuable models to study normal and aberrant EWS function upon reintroduction into the tumor cells.
| Original language | English |
|---|---|
| Pages (from-to) | 5992-5997 |
| Number of pages | 6 |
| Journal | Cancer Research |
| Volume | 61 |
| Issue number | 16 |
| Publication status | Published - Aug 15 2001 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
The EWS protein is dispensable for Ewing tumor growth. / Kovar, H.; Jug, G.; Hattinger, C.; Spahn, L.; Aryee, D. N T; Ambros, P. F.; Zoubek, A.; Gadner, H.
In: Cancer Research, Vol. 61, No. 16, 15.08.2001, p. 5992-5997.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The EWS protein is dispensable for Ewing tumor growth
AU - Kovar, H.
AU - Jug, G.
AU - Hattinger, C.
AU - Spahn, L.
AU - Aryee, D. N T
AU - Ambros, P. F.
AU - Zoubek, A.
AU - Gadner, H.
PY - 2001/8/15
Y1 - 2001/8/15
N2 - EWS encodes a ubiquitously expressed RNA binding protein with largely unknown function. In Ewing sarcoma family tumors (EFT), one allele is rearranged with an ETS gene. This is the first description of an EFT with a complete EWS deficiency in the presence of two copies of a rearranged chromosome 22 carrying an interstitial EWS-FL11 translocation. Absence of EWS protein suggested that it is dispensable for EFT growth. By sequencing of EWS cDNA from unrelated EFTs, we excluded inactivation of EWS as a general mechanism in EFT pathogenesis. Rather, EWS was found to be uniformly expressed in two splicing variants of similar abundaney, EWSα and EWSβ, which differ in a single amino acid. Three EWS negative cell lines were established, which will serve as valuable models to study normal and aberrant EWS function upon reintroduction into the tumor cells.
AB - EWS encodes a ubiquitously expressed RNA binding protein with largely unknown function. In Ewing sarcoma family tumors (EFT), one allele is rearranged with an ETS gene. This is the first description of an EFT with a complete EWS deficiency in the presence of two copies of a rearranged chromosome 22 carrying an interstitial EWS-FL11 translocation. Absence of EWS protein suggested that it is dispensable for EFT growth. By sequencing of EWS cDNA from unrelated EFTs, we excluded inactivation of EWS as a general mechanism in EFT pathogenesis. Rather, EWS was found to be uniformly expressed in two splicing variants of similar abundaney, EWSα and EWSβ, which differ in a single amino acid. Three EWS negative cell lines were established, which will serve as valuable models to study normal and aberrant EWS function upon reintroduction into the tumor cells.
UR - http://www.scopus.com/inward/record.url?scp=0035881569&partnerID=8YFLogxK
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M3 - Article
VL - 61
SP - 5992
EP - 5997
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 16
ER -