The expansion of CD8 lymphocytes using T cell receptor variable gene products during HIV infection

P. De Paoli, C. Caffau, M. D'Andrea, P. Ceolin, C. Simonelli, U. Tirelli, G. Santini

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Fresh peripheral blood was obtained from HIV-infected subjects and from age-matched healthy controls. Single-, two- and three-colour flow cytometry was performed using FITC-labelled MoAbs directed against the following TCR Vβ subfamilies: V2, V3, V5a, V5b, V5c, V6, V8, V12, V13.3, V17, V19, alone or in combination with PE-labelled CD4, CD8, HLA-DR, CD28, CD38 and with Peridinin-chlorophyll A protein (PerCP)-conjugated CD8. The percentages of each Vβ subfamily did not differ in HIV+ patients compared with healthy controls. However, we were able to find in four patients (one CDC group II, one group III and two group IV) an expansion of a TCR Vβ subfamily (V3 in two, V5a and V19 in one patient). These cells were mainly CD8+. Three-colour flow cytometry allowed us to define that the expanded Vβ+, CD8+ T lymphocytes were characterized by the low/intermediate expression of activation markers (HLA-DR, CD28, CD38). In some HIV+ patients there is an expansion of T cells expressing a TCR Vβ subfamily; the nature of this expansion may be related to factors that are still unknown, such as the genetic background of each individual and the antigenic specificity of T cells. These populations may be relevant in the host response to the virus and in disease progression.

Original languageEnglish
Pages (from-to)486-489
Number of pages4
JournalClinical and Experimental Immunology
Volume94
Issue number3
Publication statusPublished - 1993

Fingerprint

T-Cell Receptor Genes
HIV Infections
Lymphocytes
Color
HIV
HLA-DR Antigens
T-Lymphocytes
Flow Cytometry
Fluorescein-5-isothiocyanate
Virus Diseases
Chlorophyll
Centers for Disease Control and Prevention (U.S.)
Disease Progression
Epitopes
Population
Proteins

Keywords

  • CD8
  • HIV-1
  • T cell receptor subfamilies

ASJC Scopus subject areas

  • Immunology

Cite this

De Paoli, P., Caffau, C., D'Andrea, M., Ceolin, P., Simonelli, C., Tirelli, U., & Santini, G. (1993). The expansion of CD8 lymphocytes using T cell receptor variable gene products during HIV infection. Clinical and Experimental Immunology, 94(3), 486-489.

The expansion of CD8 lymphocytes using T cell receptor variable gene products during HIV infection. / De Paoli, P.; Caffau, C.; D'Andrea, M.; Ceolin, P.; Simonelli, C.; Tirelli, U.; Santini, G.

In: Clinical and Experimental Immunology, Vol. 94, No. 3, 1993, p. 486-489.

Research output: Contribution to journalArticle

De Paoli, P, Caffau, C, D'Andrea, M, Ceolin, P, Simonelli, C, Tirelli, U & Santini, G 1993, 'The expansion of CD8 lymphocytes using T cell receptor variable gene products during HIV infection', Clinical and Experimental Immunology, vol. 94, no. 3, pp. 486-489.
De Paoli, P. ; Caffau, C. ; D'Andrea, M. ; Ceolin, P. ; Simonelli, C. ; Tirelli, U. ; Santini, G. / The expansion of CD8 lymphocytes using T cell receptor variable gene products during HIV infection. In: Clinical and Experimental Immunology. 1993 ; Vol. 94, No. 3. pp. 486-489.
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