TY - JOUR
T1 - The Expression of CD90/Thy-1 in Hepatocellular Carcinoma
T2 - An In Vivo and In Vitro Study
AU - Sukowati, Caecilia Hapsari Ceriapuri
AU - Anfuso, Beatrice
AU - Torre, Giuliano
AU - Francalanci, Paola
AU - Crocè, Lory Saveria
AU - Tiribelli, Claudio
PY - 2013/10/8
Y1 - 2013/10/8
N2 - Although the CD90 (Thy-1) was proposed as biomarker of several tumors and cancer stem cells, the involvement of this molecule in the progression of hepatocellular carcinoma (HCC) and other less frequent hepatic neoplasms is still undefined. The distribution of CD90 was investigated both in in vivo (human tissues samples) and in vitro (human HCC cell line JHH-6). A total of 67 liver tumors were analyzed: 51 HCC, 6 cholangiocarcinoma and 10 hepatoblastoma. In all cases, paired tissue sample of both the tumor and cirrhotic liver was available. Hepatic tissue obtained in 12 healthy livers was used as control. CD90 gene expression was studied by RT-qPCR, protein expression was assessed by quantitative Western Blot, immunofluorescence and flow cytometry. The CD90 expression analysis showed a significant increment in tumor compared to both its paired cirrhotic tissue and normal liver (p+ compared to CD90- cells (p+ subpopulation while the multidrug resistance protein 1 showed an opposite behavior. Collectively, these results point to the importance of CD90 in the HCC.
AB - Although the CD90 (Thy-1) was proposed as biomarker of several tumors and cancer stem cells, the involvement of this molecule in the progression of hepatocellular carcinoma (HCC) and other less frequent hepatic neoplasms is still undefined. The distribution of CD90 was investigated both in in vivo (human tissues samples) and in vitro (human HCC cell line JHH-6). A total of 67 liver tumors were analyzed: 51 HCC, 6 cholangiocarcinoma and 10 hepatoblastoma. In all cases, paired tissue sample of both the tumor and cirrhotic liver was available. Hepatic tissue obtained in 12 healthy livers was used as control. CD90 gene expression was studied by RT-qPCR, protein expression was assessed by quantitative Western Blot, immunofluorescence and flow cytometry. The CD90 expression analysis showed a significant increment in tumor compared to both its paired cirrhotic tissue and normal liver (p+ compared to CD90- cells (p+ subpopulation while the multidrug resistance protein 1 showed an opposite behavior. Collectively, these results point to the importance of CD90 in the HCC.
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U2 - 10.1371/journal.pone.0076830
DO - 10.1371/journal.pone.0076830
M3 - Article
C2 - 24116172
AN - SCOPUS:84885157812
VL - 8
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 10
M1 - e76830
ER -