The expression of innate immunity genes in Italian Crohn disease patients

M. Milanese, L. Segat, N. Marziliano, Sergio Crovella

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Crohn' disease (CD) is a chronic idiopathic inflammatory bowel disease characterized by the interaction of both hereditary and environmental factors. Intestinal flora and pathogens such as bacteria, viruses and fungi, are thought to be the first step leading to an inflammatory status, which is subsequently amplified in genetically susceptible patients thus triggering the disease. Since the innate immune system is believed to be very important in regulating the flora of the gastrointestinal tract, we decided to study the influence of two important molecules of the innate immune system in CD. Frozen intestinal biopsies from 49 Crohn patients and 10 healthy individuals were collected at the gastroenterology unit of Children's Hospital Burlo Garofolo in Trieste and innate immunity gene expression was evaluated by using both in situ RT-PCR and quantitative PCR. We have analyzed the expression and localization of both MBL2 and DEFB1 genes in intestinal biopsies of Italian Crohn patients by in situ RT-PCR and quantitative PCR. DEFB1 is expressed equally in all subjects. Importantly, MBL2 transcripts were upregulated in CD patients compared to healthy controls. MBL2 expression in controls is normally extremely low, detectable only by quantitative PCR with a Taqman probe. We demonstrated the MBL2 and DEFB1 expression in intestinal biopsies of patients suffering from CD. Our results showed that the MBL2 gene is expressed by cells in the basal lamina, whilst DEFB1 is expressed by epithelial cells.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalEuropean journal of histochemistry : EJH
Volume51
Issue number3
Publication statusPublished - Jul 2007

Fingerprint

Crohn disease
Innate Immunity
Crohn Disease
Polymerase Chain Reaction
biopsy
quantitative polymerase chain reaction
Genes
Biopsy
genes
Immune System
reverse transcriptase polymerase chain reaction
Hospital Units
inflammatory bowel disease
laminae (animals)
Gastroenterology
Inflammatory Bowel Diseases
intestinal microorganisms
Basement Membrane
gastrointestinal system
Gastrointestinal Tract

Keywords

  • Crohn disease
  • DEFB1
  • Gene expression
  • Innate immunity
  • MBL2

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Plant Science
  • Biophysics
  • Cell Biology
  • Developmental Biology
  • Molecular Biology
  • Anatomy
  • Histology

Cite this

The expression of innate immunity genes in Italian Crohn disease patients. / Milanese, M.; Segat, L.; Marziliano, N.; Crovella, Sergio.

In: European journal of histochemistry : EJH, Vol. 51, No. 3, 07.2007, p. 199-202.

Research output: Contribution to journalArticle

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