The expression of Met/hepatocyte growth factor receptor gene in giant cell tumors of bone and other benign musculoskeletal tumors

Riccardo Ferracini, Katia Scotlandi, Elga Cagliero, Francesca Acquarone, Martina Olivero, Jay Wunder, Nicola Baldini

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15 Citations (Scopus)

Abstract

Overexpression of the hepatocyte growth factor receptor (Met/HGF receptor), a transmembrane tyrosine kinase encoded by the MET proto-oncogene, is involved in transformation and invasive behavior of human carcinomas and sarcomas. We have previously found that bone sarcomas express high levels of Met/HGF receptor while in some cases the ligand HGF is co-expressed with the receptor, activating an autocrine loop. In this study, we analyzed 40 biopsy samples of a collection of giant cell tumors and other rare benign tumors of bone for expression of the MET proto-oncogene. These included nonossifying fibromas, osteoblastomas, desmoplastic fibromas of bone, chondroblastomas, and giant cell tumors of bone. Snap frozen samples were tested for the MET and HGF gene expression by immuno-histochemistry and Western blotting with anti-MET antibodies and RT-PCR. Over 50% of all cases scored positive for MET expression being constantly positive in recurrent or locally aggressive lesions. Sporadic co-expression of the Met/HGF receptor and ligand is also demonstrated. Met/HGF receptor expression in benign bone neoplasms suggests its early involvement in sarcomagenesis. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)191-196
Number of pages6
JournalJournal of Cellular Physiology
Volume184
Issue number2
DOIs
Publication statusPublished - 2000

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Giant Cell Tumor of Bone
Proto-Oncogene Proteins c-met
Tumors
Bone
Genes
Proto-Oncogenes
Neoplasms
Bone and Bones
Sarcoma
Desmoplastic Fibroma
Chondroblastoma
Osteoblastoma
Ligands
Giant Cell Tumors
Bone Neoplasms
Fibroma
Biopsy
Gene expression
Protein-Tyrosine Kinases
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

The expression of Met/hepatocyte growth factor receptor gene in giant cell tumors of bone and other benign musculoskeletal tumors. / Ferracini, Riccardo; Scotlandi, Katia; Cagliero, Elga; Acquarone, Francesca; Olivero, Martina; Wunder, Jay; Baldini, Nicola.

In: Journal of Cellular Physiology, Vol. 184, No. 2, 2000, p. 191-196.

Research output: Contribution to journalArticle

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AU - Olivero, Martina

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AU - Baldini, Nicola

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