The expression of riboflavin transporters in human colorectal cancer

Valeria Tutino, Marianna Loredana Defrancesco, Maria Tolomeo, Valentina De Nunzio, Dionigi Lorusso, Didier Paleni, Maria Gabriella Caruso, Maria Notarnicola, Maria Barile

Research output: Contribution to journalArticle

Abstract

Background/Aim: Riboflavin transport in enterocytes is mediated by three translocators: RFVT3 located on the apical membrane, and RFVT1 and RFVT2 on the basolateral membrane. The aim of this study was to investigate whether the expression levels of RFVTs are altered in human colorectal cancer (CRC). Materials and Methods: In human colon adenocarcinoma cell lines (CaCo2, DLD-1, HT-29) and in tissues of patients with CRC, gene and protein expression levels were evaluated by real time-polymerase chain reaction and western blotting. Intracellular flavin content was determined by high-performance liquid chromatography. Results: RFVT3 and RFVT2 gene and protein expression levels were higher in DLD-1 and HT-29 compared to Caco2 cells. In HT-29 cells, the RFVT1 protein level was drastically lower. These differences are presumably responsible for the higher total flavin content in DLD-1 and HT-29 cells. In tumor tissues of patients with CRC, RFVT1 content was reduced at both protein and mRNA levels compared to normal mucosa. RFVT3 and RFVT2 gene expression levels were increased, while protein expression was reduced, with a small reduction in riboflavin amount. Conclusion: This study provides first evidence that transcription/translation of RFVTs are profoundly altered in CRC.

Original languageEnglish
Pages (from-to)2659-2667
Number of pages9
JournalAnticancer Research
Volume38
Issue number5
DOIs
Publication statusPublished - May 1 2018

Keywords

  • Cell lines
  • Colorectal cancer
  • Flavin cofactor homeostasis
  • Riboflavin
  • Riboflavin transporters

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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