The expression of the non-receptor tyrosine kinases Arg and c-abl is differently modulated in B lymphoid cells at different stages of differentiation

Cristina Bianchi, Ivan Muradore, Matteo Corizzato, Giorgia Cornacchini, Luca Beretta, Eugenio Erba, Ugo Del Monte, Roberto A. Perego

Research output: Contribution to journalArticle

Abstract

The products of the human ARG gene and the human ABL gene characterize the Abelson family of non-receptor tyrosine protein kinases. Both genes are ubiquitously expressed. The interactions of these two similar protein kinases are still not well known, although it has been suggested that they could cooperate, with redundant actions, to provide intracellular signals in the cells. Lymphopenia occurs in mice with homozygous disruption of c-abl, indicating that in certain tissues Arg is unable to substitute c-abl functions. In B and T lymphoid cell lines at different stages of differentiation, we studied, by a reverse transcriptase-competitive polymerase chain reaction and Western blotting, Arg and c-abl in order to evaluate whether the expression pattern of the two genes could give insight as to why they do not exhibit overlapping roles in lymphocytes and whether the product levels of the two genes are related to lymphoid differentiation. The data showed that their expression is differently modified in lymphoid B cell lines. The highest Arg transcript and protein levels are in the mature B cells.

Original languageEnglish
Pages (from-to)216-222
Number of pages7
JournalFEBS Letters
Volume527
Issue number1-3
DOIs
Publication statusPublished - Sep 11 2002

    Fingerprint

Keywords

  • Arg
  • C-abl
  • Competitive polymerase chain reaction
  • Differentiation
  • Lymphoid cell line
  • Non-receptor tyrosine kinase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Bianchi, C., Muradore, I., Corizzato, M., Cornacchini, G., Beretta, L., Erba, E., Del Monte, U., & A. Perego, R. (2002). The expression of the non-receptor tyrosine kinases Arg and c-abl is differently modulated in B lymphoid cells at different stages of differentiation. FEBS Letters, 527(1-3), 216-222. https://doi.org/10.1016/S0014-5793(02)03233-7