The FHIT gene at 3p14.2 is abnormal in lung cancer

Gabriella Sozzi; Maria Luisa Veronese; Massimo Negrini; Raffaele Baffa; Maria Grazia Cotticelli; Hiroshi Inoue; Silvana Tornielli; Silvana Pilotti; Laura De Gregorio; Ugo Pastorino; Marco A. Pierotti; Masataka Ohta; Kay Huebner; Carlo M. Croce

doi:10.1016/S0092-8674(00)81078-8

The FHIT gene at 3p14.2 is abnormal in lung cancer

Gabriella Sozzi, Maria Luisa Veronese, Massimo Negrini, Raffaele Baffa, Maria Grazia Cotticelli, Hiroshi Inoue, Silvana Tornielli, Silvana Pilotti, Laura De Gregorio, Ugo Pastorino, Marco A. Pierotti, Masataka Ohta, Kay Huebner, Carlo M. Croce

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article

Abstract

To determine the role of the FHIT gene, which encompasses the fragile site at 3p14.2, we analyzed 59 tumors of the small cell and non-small cell type by reverse transcription of FHIT mRNA, followed by PCR amplification and sequencing of products. Allelic losses affecting the gene were evaluated by microsatellite polymorphism analysis and genomic alterations by hybridization using cDNA and genomic probes. Small cell lung tumors (80%) and non-small cell lung cancers (40%) showed abnormalities in RNA transcripts of FHIT, and 76% of the tumors exhibited loss of FHIT alleles. Abnormal lung tumor transcripts lack two or more exons of the FHIT gene. Small cell lung cancer tumors and cell lines were analyzed by Southern blotting and showed rearranged BamHI fragments. These data suggest a critical role of the FHIT gene in lung carcinogenesis.

Original language	English
Pages (from-to)	17-26
Number of pages	10
Journal	Cell
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/S0092-8674(00)81078-8
Publication status	Published - Apr 5 1996

ASJC Scopus subject areas

Cell Biology
Molecular Biology

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Sozzi, G., Veronese, M. L., Negrini, M., Baffa, R., Cotticelli, M. G., Inoue, H., Tornielli, S., Pilotti, S., De Gregorio, L., Pastorino, U., Pierotti, M. A., Ohta, M., Huebner, K., & Croce, C. M. (1996). The FHIT gene at 3p14.2 is abnormal in lung cancer. Cell, 85(1), 17-26. https://doi.org/10.1016/S0092-8674(00)81078-8

The FHIT gene at 3p14.2 is abnormal in lung cancer. / Sozzi, Gabriella; Veronese, Maria Luisa; Negrini, Massimo; Baffa, Raffaele; Cotticelli, Maria Grazia; Inoue, Hiroshi; Tornielli, Silvana; Pilotti, Silvana; De Gregorio, Laura; Pastorino, Ugo; Pierotti, Marco A.; Ohta, Masataka; Huebner, Kay; Croce, Carlo M.

In: Cell, Vol. 85, No. 1, 05.04.1996, p. 17-26.

Research output: Contribution to journal › Article

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title = "The FHIT gene at 3p14.2 is abnormal in lung cancer",

abstract = "To determine the role of the FHIT gene, which encompasses the fragile site at 3p14.2, we analyzed 59 tumors of the small cell and non-small cell type by reverse transcription of FHIT mRNA, followed by PCR amplification and sequencing of products. Allelic losses affecting the gene were evaluated by microsatellite polymorphism analysis and genomic alterations by hybridization using cDNA and genomic probes. Small cell lung tumors (80%) and non-small cell lung cancers (40%) showed abnormalities in RNA transcripts of FHIT, and 76% of the tumors exhibited loss of FHIT alleles. Abnormal lung tumor transcripts lack two or more exons of the FHIT gene. Small cell lung cancer tumors and cell lines were analyzed by Southern blotting and showed rearranged BamHI fragments. These data suggest a critical role of the FHIT gene in lung carcinogenesis.",

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AU - De Gregorio, Laura

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