The FHIT gene at 3p14.2 is abnormal in lung cancer

Gabriella Sozzi, Maria Luisa Veronese, Massimo Negrini, Raffaele Baffa, Maria Grazia Cotticelli, Hiroshi Inoue, Silvana Tornielli, Silvana Pilotti, Laura De Gregorio, Ugo Pastorino, Marco A. Pierotti, Masataka Ohta, Kay Huebner, Carlo M. Croce

Research output: Contribution to journalArticle

Abstract

To determine the role of the FHIT gene, which encompasses the fragile site at 3p14.2, we analyzed 59 tumors of the small cell and non-small cell type by reverse transcription of FHIT mRNA, followed by PCR amplification and sequencing of products. Allelic losses affecting the gene were evaluated by microsatellite polymorphism analysis and genomic alterations by hybridization using cDNA and genomic probes. Small cell lung tumors (80%) and non-small cell lung cancers (40%) showed abnormalities in RNA transcripts of FHIT, and 76% of the tumors exhibited loss of FHIT alleles. Abnormal lung tumor transcripts lack two or more exons of the FHIT gene. Small cell lung cancer tumors and cell lines were analyzed by Southern blotting and showed rearranged BamHI fragments. These data suggest a critical role of the FHIT gene in lung carcinogenesis.

Original languageEnglish
Pages (from-to)17-26
Number of pages10
JournalCell
Volume85
Issue number1
DOIs
Publication statusPublished - Apr 5 1996

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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  • Cite this

    Sozzi, G., Veronese, M. L., Negrini, M., Baffa, R., Cotticelli, M. G., Inoue, H., Tornielli, S., Pilotti, S., De Gregorio, L., Pastorino, U., Pierotti, M. A., Ohta, M., Huebner, K., & Croce, C. M. (1996). The FHIT gene at 3p14.2 is abnormal in lung cancer. Cell, 85(1), 17-26. https://doi.org/10.1016/S0092-8674(00)81078-8