The Fragile X Protein binds mRNAs involved in cancer progression and modulates metastasis formation

Rossella Lucá, Michele Averna, Francesca Zalfa, Manuela Vecchi, Fabrizio Bianchi, Giorgio La Fata, Franca Del Nonno, Roberta Nardacci, Marco Bianchi, Paolo Nuciforo, Sebastian Munck, Paola Parrella, Rute Moura, Emanuela Signori, Robert Alston, Anna Kuchnio, Maria Giulia Farace, Vito Michele Fazio, Mauro Piacentini, Bart De StrooperTilmann Achsel, Giovanni Neri, Patrick Neven, D. Gareth Evans, Peter Carmeliet, Massimiliano Mazzone, Claudia Bagni

Research output: Contribution to journalArticle

Abstract

The role of the fragile X mental retardation protein (FMRP) is well established in brain, where its absence leads to the fragile X syndrome (FXS). FMRP is almost ubiquitously expressed, suggesting that, in addition to its effects in brain, it may have fundamental roles in other organs. There is evidence that FMRP expression can be linked to cancer. FMR1 mRNA, encoding FMRP, is overexpressed in hepatocellular carcinoma cells. A decreased risk of cancer has been reported in patients with FXS while a patient-case with FXS showed an unusual decrease of tumour brain invasiveness. However, a role for FMRP in regulating cancer biology, if any, remains unknown. We show here that FMRP and FMR1 mRNA levels correlate with prognostic indicators of aggressive breast cancer, lung metastases probability and triple negative breast cancer (TNBC). We establish that FMRP overexpression in murine breast primary tumours enhances lung metastasis while its reduction has the opposite effect regulating cell spreading and invasion. FMRP binds mRNAs involved in epithelial mesenchymal transition (EMT) and invasion including E-cadherin and Vimentin mRNAs, hallmarks of EMT and cancer progression.

Original languageEnglish
Pages (from-to)1523-1536
Number of pages14
JournalEMBO Molecular Medicine
Volume5
Issue number10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Fragile X Mental Retardation Protein
Neoplasm Metastasis
Messenger RNA
Fragile X Syndrome
Neoplasms
Proteins
Epithelial-Mesenchymal Transition
Triple Negative Breast Neoplasms
Breast Neoplasms
Brain
Vimentin
Cadherins
Brain Neoplasms
Hepatocellular Carcinoma
Lung Neoplasms
Lung

Keywords

  • Cell invasion
  • EMT
  • FMRP
  • MRNA metabolism
  • TNBC

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

The Fragile X Protein binds mRNAs involved in cancer progression and modulates metastasis formation. / Lucá, Rossella; Averna, Michele; Zalfa, Francesca; Vecchi, Manuela; Bianchi, Fabrizio; Fata, Giorgio La; Del Nonno, Franca; Nardacci, Roberta; Bianchi, Marco; Nuciforo, Paolo; Munck, Sebastian; Parrella, Paola; Moura, Rute; Signori, Emanuela; Alston, Robert; Kuchnio, Anna; Farace, Maria Giulia; Fazio, Vito Michele; Piacentini, Mauro; De Strooper, Bart; Achsel, Tilmann; Neri, Giovanni; Neven, Patrick; Evans, D. Gareth; Carmeliet, Peter; Mazzone, Massimiliano; Bagni, Claudia.

In: EMBO Molecular Medicine, Vol. 5, No. 10, 10.2013, p. 1523-1536.

Research output: Contribution to journalArticle

Lucá, R, Averna, M, Zalfa, F, Vecchi, M, Bianchi, F, Fata, GL, Del Nonno, F, Nardacci, R, Bianchi, M, Nuciforo, P, Munck, S, Parrella, P, Moura, R, Signori, E, Alston, R, Kuchnio, A, Farace, MG, Fazio, VM, Piacentini, M, De Strooper, B, Achsel, T, Neri, G, Neven, P, Evans, DG, Carmeliet, P, Mazzone, M & Bagni, C 2013, 'The Fragile X Protein binds mRNAs involved in cancer progression and modulates metastasis formation', EMBO Molecular Medicine, vol. 5, no. 10, pp. 1523-1536. https://doi.org/10.1002/emmm.201302847
Lucá, Rossella ; Averna, Michele ; Zalfa, Francesca ; Vecchi, Manuela ; Bianchi, Fabrizio ; Fata, Giorgio La ; Del Nonno, Franca ; Nardacci, Roberta ; Bianchi, Marco ; Nuciforo, Paolo ; Munck, Sebastian ; Parrella, Paola ; Moura, Rute ; Signori, Emanuela ; Alston, Robert ; Kuchnio, Anna ; Farace, Maria Giulia ; Fazio, Vito Michele ; Piacentini, Mauro ; De Strooper, Bart ; Achsel, Tilmann ; Neri, Giovanni ; Neven, Patrick ; Evans, D. Gareth ; Carmeliet, Peter ; Mazzone, Massimiliano ; Bagni, Claudia. / The Fragile X Protein binds mRNAs involved in cancer progression and modulates metastasis formation. In: EMBO Molecular Medicine. 2013 ; Vol. 5, No. 10. pp. 1523-1536.
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AU - Neri, Giovanni

AU - Neven, Patrick

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