TY - JOUR
T1 - The frequency of interleukin-10- and interleukin-5-secreting CD4 + T cells correlates to tolerance of transplanted lung
AU - Marone Bianco, A.
AU - Solari, N.
AU - Miserere, S.
AU - Pellegrini, C.
AU - Vitulo, P.
AU - Pozzi, E.
AU - Fietta, A.
AU - Meloni, F.
PY - 2005/6
Y1 - 2005/6
N2 - Posttransplant bronchiolitis obliterans syndrome (BOS) results from a chronic immunological/inflammatory insult that leads to fibro-obliteration of the lumen of the allograft airways. The functional T-cell response that is associated with graft acceptance needs to be further clarified in humans. The aim of the present study was to assess the functional activity of peripheral CD4+ T lymphocytes in nine lung transplant recipients with BOS stage II or III (mean 5.4 years after transplant), in seven lung patients with stable clinical conditions (3.4 years posttransplant); and in six normal controls. Peripheral CD4+ T cells, obtained by magnetic bead vs negative purification, were studied using a computer-assisted enzyme-linked immunospot assay (ELISPOT) to assess the number of IFN-γ-, interleukin (IL)5-, and IL10-γ-producing cells (no./106 CD4+ cells) after allogeneic stimulation. The frequencies of IFN-γ-producing CD4+ cells did not change significantly in stable patients compared to those with BOS. Interestingly in BOS, the number of IL5- and IL10-producing cells was significantly lower than in stable patients (P ≤ .05), suggesting a possible role of these Th2 cytokines in the modulation of graft tolerance.
AB - Posttransplant bronchiolitis obliterans syndrome (BOS) results from a chronic immunological/inflammatory insult that leads to fibro-obliteration of the lumen of the allograft airways. The functional T-cell response that is associated with graft acceptance needs to be further clarified in humans. The aim of the present study was to assess the functional activity of peripheral CD4+ T lymphocytes in nine lung transplant recipients with BOS stage II or III (mean 5.4 years after transplant), in seven lung patients with stable clinical conditions (3.4 years posttransplant); and in six normal controls. Peripheral CD4+ T cells, obtained by magnetic bead vs negative purification, were studied using a computer-assisted enzyme-linked immunospot assay (ELISPOT) to assess the number of IFN-γ-, interleukin (IL)5-, and IL10-γ-producing cells (no./106 CD4+ cells) after allogeneic stimulation. The frequencies of IFN-γ-producing CD4+ cells did not change significantly in stable patients compared to those with BOS. Interestingly in BOS, the number of IL5- and IL10-producing cells was significantly lower than in stable patients (P ≤ .05), suggesting a possible role of these Th2 cytokines in the modulation of graft tolerance.
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U2 - 10.1016/j.transproceed.2005.03.050
DO - 10.1016/j.transproceed.2005.03.050
M3 - Article
C2 - 15964392
AN - SCOPUS:21844452668
VL - 37
SP - 2255
EP - 2256
JO - Transplantation Proceedings
JF - Transplantation Proceedings
SN - 0041-1345
IS - 5
ER -