The generation of nitric oxide participates in γIFN-induced MHC class II antigen expression by cultured astrocytoma cells

M. Colasanti, V. Mollace, E. Cundari, R. Massoud, G. Nistico, G. M. Lauro

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The effects of interferon gamma (γIFN) on the MHCII antigen expression by human cultured astrocytoma cells were investigated. The co-incubation of γIFN with T67 astrocytoma cells produced a dose-dependent increase of MHCII antigen expression as evaluated by flow cytometric (FACS) analysis and confocal laser microscopy analysis. The number of NHCII molecules expressed by γIFN-pretreated astrocytoma cells was reduced by co-incubation with Nω-nitro-L-arginine methyl ester (L-NAME), a selective inhibitor of the nitric oxide (NO)-synthesizing enzyme. In addition, methylene blue, which inhibits the biological activity of NO acting at the guanylate cyclase level, strongly antagonized the MHCII antigen expression on astrocytoma cells induced by γIFN. Furthermore, γIFN increased the activity of the inducible isoform of NO-synthase as well as the concentration of nitrite, one of the breakdown products of NO and the antiplatelet activity of astrocytoma cells. In conclusion, the present data show that γIFN increases the synthesis and release of NO by cultured astrocytoma cells and this could co-participate in the MHCII antigen expression by this cell type. Therefore, the generation of NO by cultured astrocytoma cells may represent an important step in the development of the immunocompetent activity of astrocytes.

Original languageEnglish
Pages (from-to)763-771
Number of pages9
JournalInternational Journal of Immunopharmacology
Volume15
Issue number6
DOIs
Publication statusPublished - 1993

Fingerprint

Histocompatibility Antigens Class II
Astrocytoma
Interferon-gamma
Cultured Cells
Nitric Oxide
Antigens
Confocal Microscopy
Guanylate Cyclase
Methylene Blue
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase Type II
Nitrites
Astrocytes
Protein Isoforms
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

The generation of nitric oxide participates in γIFN-induced MHC class II antigen expression by cultured astrocytoma cells. / Colasanti, M.; Mollace, V.; Cundari, E.; Massoud, R.; Nistico, G.; Lauro, G. M.

In: International Journal of Immunopharmacology, Vol. 15, No. 6, 1993, p. 763-771.

Research output: Contribution to journalArticle

Colasanti, M. ; Mollace, V. ; Cundari, E. ; Massoud, R. ; Nistico, G. ; Lauro, G. M. / The generation of nitric oxide participates in γIFN-induced MHC class II antigen expression by cultured astrocytoma cells. In: International Journal of Immunopharmacology. 1993 ; Vol. 15, No. 6. pp. 763-771.
@article{096aa90b48e94b2bab651e7130453d6b,
title = "The generation of nitric oxide participates in γIFN-induced MHC class II antigen expression by cultured astrocytoma cells",
abstract = "The effects of interferon gamma (γIFN) on the MHCII antigen expression by human cultured astrocytoma cells were investigated. The co-incubation of γIFN with T67 astrocytoma cells produced a dose-dependent increase of MHCII antigen expression as evaluated by flow cytometric (FACS) analysis and confocal laser microscopy analysis. The number of NHCII molecules expressed by γIFN-pretreated astrocytoma cells was reduced by co-incubation with Nω-nitro-L-arginine methyl ester (L-NAME), a selective inhibitor of the nitric oxide (NO)-synthesizing enzyme. In addition, methylene blue, which inhibits the biological activity of NO acting at the guanylate cyclase level, strongly antagonized the MHCII antigen expression on astrocytoma cells induced by γIFN. Furthermore, γIFN increased the activity of the inducible isoform of NO-synthase as well as the concentration of nitrite, one of the breakdown products of NO and the antiplatelet activity of astrocytoma cells. In conclusion, the present data show that γIFN increases the synthesis and release of NO by cultured astrocytoma cells and this could co-participate in the MHCII antigen expression by this cell type. Therefore, the generation of NO by cultured astrocytoma cells may represent an important step in the development of the immunocompetent activity of astrocytes.",
author = "M. Colasanti and V. Mollace and E. Cundari and R. Massoud and G. Nistico and Lauro, {G. M.}",
year = "1993",
doi = "10.1016/0192-0561(93)90150-W",
language = "English",
volume = "15",
pages = "763--771",
journal = "International Journal of Immunopharmacology",
issn = "0192-0561",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - The generation of nitric oxide participates in γIFN-induced MHC class II antigen expression by cultured astrocytoma cells

AU - Colasanti, M.

AU - Mollace, V.

AU - Cundari, E.

AU - Massoud, R.

AU - Nistico, G.

AU - Lauro, G. M.

PY - 1993

Y1 - 1993

N2 - The effects of interferon gamma (γIFN) on the MHCII antigen expression by human cultured astrocytoma cells were investigated. The co-incubation of γIFN with T67 astrocytoma cells produced a dose-dependent increase of MHCII antigen expression as evaluated by flow cytometric (FACS) analysis and confocal laser microscopy analysis. The number of NHCII molecules expressed by γIFN-pretreated astrocytoma cells was reduced by co-incubation with Nω-nitro-L-arginine methyl ester (L-NAME), a selective inhibitor of the nitric oxide (NO)-synthesizing enzyme. In addition, methylene blue, which inhibits the biological activity of NO acting at the guanylate cyclase level, strongly antagonized the MHCII antigen expression on astrocytoma cells induced by γIFN. Furthermore, γIFN increased the activity of the inducible isoform of NO-synthase as well as the concentration of nitrite, one of the breakdown products of NO and the antiplatelet activity of astrocytoma cells. In conclusion, the present data show that γIFN increases the synthesis and release of NO by cultured astrocytoma cells and this could co-participate in the MHCII antigen expression by this cell type. Therefore, the generation of NO by cultured astrocytoma cells may represent an important step in the development of the immunocompetent activity of astrocytes.

AB - The effects of interferon gamma (γIFN) on the MHCII antigen expression by human cultured astrocytoma cells were investigated. The co-incubation of γIFN with T67 astrocytoma cells produced a dose-dependent increase of MHCII antigen expression as evaluated by flow cytometric (FACS) analysis and confocal laser microscopy analysis. The number of NHCII molecules expressed by γIFN-pretreated astrocytoma cells was reduced by co-incubation with Nω-nitro-L-arginine methyl ester (L-NAME), a selective inhibitor of the nitric oxide (NO)-synthesizing enzyme. In addition, methylene blue, which inhibits the biological activity of NO acting at the guanylate cyclase level, strongly antagonized the MHCII antigen expression on astrocytoma cells induced by γIFN. Furthermore, γIFN increased the activity of the inducible isoform of NO-synthase as well as the concentration of nitrite, one of the breakdown products of NO and the antiplatelet activity of astrocytoma cells. In conclusion, the present data show that γIFN increases the synthesis and release of NO by cultured astrocytoma cells and this could co-participate in the MHCII antigen expression by this cell type. Therefore, the generation of NO by cultured astrocytoma cells may represent an important step in the development of the immunocompetent activity of astrocytes.

UR - http://www.scopus.com/inward/record.url?scp=0027293442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027293442&partnerID=8YFLogxK

U2 - 10.1016/0192-0561(93)90150-W

DO - 10.1016/0192-0561(93)90150-W

M3 - Article

C2 - 7691770

AN - SCOPUS:0027293442

VL - 15

SP - 763

EP - 771

JO - International Journal of Immunopharmacology

JF - International Journal of Immunopharmacology

SN - 0192-0561

IS - 6

ER -