The GluR2 subunit inhibits proliferation by inactivating Src-MAPK signalling and induces apoptosis by means of caspase 3/6-dependent activation in glioma cells

Francesca Beretta, Silvia Bassani, Elena Binda, Chiara Verpelli, Lorenzo Bello, Rossella Galli, Maria Passafaro

Research output: Contribution to journalArticle

Abstract

Glioblastoma multiforme (GBM) is the most invasive and undifferentiated type of brain tumour, and so surgical interventions are ineffective. We found that GluR2 is absent in fast-growing GBM-derived tumour stem cells and high-grade glioma specimens, but is expressed in slow-growing stem cells and low-grade glioma specimens. More remarkably, GluR2 overexpression in U-87MG cells inhibits proliferation by inactivating extracellular signal-regulated kinase (ERK)1/2-Src phosphorylation and induces apoptosis. Mechanistically, we observed that the scaffold protein GRIP is essential for the effect of GluR2 on ERK-Src inactivation. These findings indicate that the absence of the GluR2 subunit favours malignancy.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalEuropean Journal of Neuroscience
Volume30
Issue number1
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Caspase 6
Glioblastoma
Glioma
Caspase 3
Apoptosis
Mitogen-Activated Protein Kinase 3
Neoplastic Stem Cells
Mitogen-Activated Protein Kinase 1
Extracellular Signal-Regulated MAP Kinases
Brain Neoplasms
Stem Cells
Phosphorylation
Cell Proliferation
Neoplasms
Proteins

Keywords

  • ERK1/2
  • Glioma specimens
  • GRIP
  • Tumour stem cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The GluR2 subunit inhibits proliferation by inactivating Src-MAPK signalling and induces apoptosis by means of caspase 3/6-dependent activation in glioma cells. / Beretta, Francesca; Bassani, Silvia; Binda, Elena; Verpelli, Chiara; Bello, Lorenzo; Galli, Rossella; Passafaro, Maria.

In: European Journal of Neuroscience, Vol. 30, No. 1, 07.2009, p. 25-34.

Research output: Contribution to journalArticle

@article{8c5192e93f1e438e8a41469222d221ae,
title = "The GluR2 subunit inhibits proliferation by inactivating Src-MAPK signalling and induces apoptosis by means of caspase 3/6-dependent activation in glioma cells",
abstract = "Glioblastoma multiforme (GBM) is the most invasive and undifferentiated type of brain tumour, and so surgical interventions are ineffective. We found that GluR2 is absent in fast-growing GBM-derived tumour stem cells and high-grade glioma specimens, but is expressed in slow-growing stem cells and low-grade glioma specimens. More remarkably, GluR2 overexpression in U-87MG cells inhibits proliferation by inactivating extracellular signal-regulated kinase (ERK)1/2-Src phosphorylation and induces apoptosis. Mechanistically, we observed that the scaffold protein GRIP is essential for the effect of GluR2 on ERK-Src inactivation. These findings indicate that the absence of the GluR2 subunit favours malignancy.",
keywords = "ERK1/2, Glioma specimens, GRIP, Tumour stem cells",
author = "Francesca Beretta and Silvia Bassani and Elena Binda and Chiara Verpelli and Lorenzo Bello and Rossella Galli and Maria Passafaro",
year = "2009",
month = "7",
doi = "10.1111/j.1460-9568.2009.06804.x",
language = "English",
volume = "30",
pages = "25--34",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - The GluR2 subunit inhibits proliferation by inactivating Src-MAPK signalling and induces apoptosis by means of caspase 3/6-dependent activation in glioma cells

AU - Beretta, Francesca

AU - Bassani, Silvia

AU - Binda, Elena

AU - Verpelli, Chiara

AU - Bello, Lorenzo

AU - Galli, Rossella

AU - Passafaro, Maria

PY - 2009/7

Y1 - 2009/7

N2 - Glioblastoma multiforme (GBM) is the most invasive and undifferentiated type of brain tumour, and so surgical interventions are ineffective. We found that GluR2 is absent in fast-growing GBM-derived tumour stem cells and high-grade glioma specimens, but is expressed in slow-growing stem cells and low-grade glioma specimens. More remarkably, GluR2 overexpression in U-87MG cells inhibits proliferation by inactivating extracellular signal-regulated kinase (ERK)1/2-Src phosphorylation and induces apoptosis. Mechanistically, we observed that the scaffold protein GRIP is essential for the effect of GluR2 on ERK-Src inactivation. These findings indicate that the absence of the GluR2 subunit favours malignancy.

AB - Glioblastoma multiforme (GBM) is the most invasive and undifferentiated type of brain tumour, and so surgical interventions are ineffective. We found that GluR2 is absent in fast-growing GBM-derived tumour stem cells and high-grade glioma specimens, but is expressed in slow-growing stem cells and low-grade glioma specimens. More remarkably, GluR2 overexpression in U-87MG cells inhibits proliferation by inactivating extracellular signal-regulated kinase (ERK)1/2-Src phosphorylation and induces apoptosis. Mechanistically, we observed that the scaffold protein GRIP is essential for the effect of GluR2 on ERK-Src inactivation. These findings indicate that the absence of the GluR2 subunit favours malignancy.

KW - ERK1/2

KW - Glioma specimens

KW - GRIP

KW - Tumour stem cells

UR - http://www.scopus.com/inward/record.url?scp=67649871267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649871267&partnerID=8YFLogxK

U2 - 10.1111/j.1460-9568.2009.06804.x

DO - 10.1111/j.1460-9568.2009.06804.x

M3 - Article

C2 - 19558602

AN - SCOPUS:67649871267

VL - 30

SP - 25

EP - 34

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 1

ER -