The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetate.

C. Soldani, M. G. Bottone, A. C. Croce, A. Fraschini, G. Bottiroli, C. Pellicciari

Research output: Contribution to journalArticle

Abstract

The aim of the present investigation was to elucidate whether the Golgi apparatus undergoes photodamage following administration of the fluorogenic substrates Rose Bengal acetate (RBAc) and irradiation at the appropriate wavelength. Human HeLa cells were treated in culture and the changes in the organization of the Golgi apparatus were studied using fluorescence confocal microscopy and electron microscopy, after immunocytochemical labeling. To see whether the cytoskeletal components primarily involved in vesicle traffic (i.e., microtubules) might also be affected, experiments of tubulin immunolabeling were performed. After treatment with RBAc and irradiation, cells were allowed to grow in drug-free medium for different times. 24 hr after irradiation, the cisternae of the Golgi apparatus became packed, and after 48-72 hr they appeared more fragmented and scattered throughout the cytoplasm; these changes in the organization of the Golgi cisternae were confirmed at electron microscopy. Interestingly enough, apoptosis was found to occur especially 48-72 h after irradiation, and apoptotic cells exhibited a dramatic fragmentation of the Golgi membranes. The immunolabeling with anti-tubulin antibody showed that microtubules were also affected by irradiation in RBAc-treated cells.

Original languageEnglish
Pages (from-to)443-448
Number of pages6
JournalEuropean journal of histochemistry : EJH
Volume48
Issue number4
Publication statusPublished - Oct 2004

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Photosensitivity Disorders
Golgi Apparatus
Golgi apparatus
irradiation
acetates
Tubulin
Microtubules
Electron Microscopy
tubulin
microtubules
electron microscopy
Fluorescent Dyes
HeLa Cells
Fluorescence Microscopy
Confocal Microscopy
Anti-Idiotypic Antibodies
cells
Cytoplasm
Apoptosis
fluorescence microscopy

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Animal Science and Zoology
  • Developmental Biology

Cite this

The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetate. / Soldani, C.; Bottone, M. G.; Croce, A. C.; Fraschini, A.; Bottiroli, G.; Pellicciari, C.

In: European journal of histochemistry : EJH, Vol. 48, No. 4, 10.2004, p. 443-448.

Research output: Contribution to journalArticle

Soldani, C, Bottone, MG, Croce, AC, Fraschini, A, Bottiroli, G & Pellicciari, C 2004, 'The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetate.', European journal of histochemistry : EJH, vol. 48, no. 4, pp. 443-448.
Soldani, C. ; Bottone, M. G. ; Croce, A. C. ; Fraschini, A. ; Bottiroli, G. ; Pellicciari, C. / The Golgi apparatus is a primary site of intracellular damage after photosensitization with Rose Bengal acetate. In: European journal of histochemistry : EJH. 2004 ; Vol. 48, No. 4. pp. 443-448.
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