The Golgi mitotic checkpoint is controlled by BARS-dependent fission of the Golgi ribbon into separate stacks in G2

Antonino Colanzi, Cristina Hidalgo Carcedo, Angela Persico, Claudia Cericola, Gabriele Turacchio, Matteo Bonazzi, Alberto Luini, Daniela Corda

Research output: Contribution to journalArticlepeer-review

Abstract

The Golgi ribbon is a complex structure of many stacks interconnected by tubules that undergo fragmentation during mitosis through a multistage process that allows correct Golgi inheritance. The fissioning protein CtBP1-S/BARS (BARS) is essential for this, and is itself required for mitotic entry: a block in Golgi fragmentation results in cell-cycle arrest in G2, defining the 'Golgi mitotic checkpoint'. Here, we clarify the precise stage of Golgi fragmentation required for mitotic entry and the role of BARS in this process. Thus, during G2, the Golgi ribbon is converted into isolated stacks by fission of interstack connecting tubules. This requires BARS and is sufficient for G2/M transition. Cells without a Golgi ribbon are independent of BARS for Golgi fragmentation and mitotic entrance. Remarkably, fibroblasts from BARS-knockout embryos have their Golgi complex divided into isolated stacks at all cell-cycle stages, bypassing the need for BARS for Golgi fragmentation. This identifies the precise stage of Golgi fragmentation and the role of BARS in the Golgi mitotic checkpoint, setting the stage for molecular analysis of this process.

Original languageEnglish
Pages (from-to)2465-2476
Number of pages12
JournalEMBO Journal
Volume26
Issue number10
DOIs
Publication statusPublished - May 16 2007

Keywords

  • BARS
  • Fission
  • G2
  • Golgi complex
  • Mitotic checkpoint

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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