The gp120 glycoprotein of human immunodeficiency virus type 1 binds to sensory ganglion neurons

Slobodan Apostolski, Terence McAlarney, Angelo Quattrini, Steven W. Levison, Gorazd Rosoklija, Alessandra Lugaressi, Massimo Corbo, Saud A. Sadiq, Seth Lederman, Arthur P. Hays, Norman Latov

Research output: Contribution to journalArticle

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Abstract

Using immunofluorescence microscopy we found that gp120 binds to the surface of rat dorsal root ganglia neurons and human neuroblastoma cells but not to rat fibroblasts or glial cells. The binding of gp120 to neurons was eliminated by pretreatment with trypsin, which removes cell-surface proteins, but not with chloroform : methanol, which removes glycolipids. As control, neuronal staining by antisulfatide antibodies was eliminated by pretreatment with chloroform : methanol but not with trypsin. The gp120 binding to neurons was also inhibited by the mouse monoclonal antibody 01, which binds to galactocerebroside and cross-reactive glycoproteins. These studies suggest that the receptor for gp120 on the surface of the dorsal root ganglia neurons is a glycoprotein. This interaction may mediate the effects of human immunodeficiency virus type 1 in sensory neuropathy.

Original languageEnglish
Pages (from-to)855-863
Number of pages9
JournalAnnals of Neurology
Volume34
Issue number6
Publication statusPublished - Dec 1993

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Sensory Ganglia
Sensory Receptor Cells
HIV-1
Glycoproteins
Neurons
Spinal Ganglia
Chloroform
Trypsin
Methanol
Glycolipids
Neuroblastoma
Fluorescence Microscopy
Neuroglia
Membrane Proteins
Fibroblasts
Monoclonal Antibodies
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Apostolski, S., McAlarney, T., Quattrini, A., Levison, S. W., Rosoklija, G., Lugaressi, A., ... Latov, N. (1993). The gp120 glycoprotein of human immunodeficiency virus type 1 binds to sensory ganglion neurons. Annals of Neurology, 34(6), 855-863.

The gp120 glycoprotein of human immunodeficiency virus type 1 binds to sensory ganglion neurons. / Apostolski, Slobodan; McAlarney, Terence; Quattrini, Angelo; Levison, Steven W.; Rosoklija, Gorazd; Lugaressi, Alessandra; Corbo, Massimo; Sadiq, Saud A.; Lederman, Seth; Hays, Arthur P.; Latov, Norman.

In: Annals of Neurology, Vol. 34, No. 6, 12.1993, p. 855-863.

Research output: Contribution to journalArticle

Apostolski, S, McAlarney, T, Quattrini, A, Levison, SW, Rosoklija, G, Lugaressi, A, Corbo, M, Sadiq, SA, Lederman, S, Hays, AP & Latov, N 1993, 'The gp120 glycoprotein of human immunodeficiency virus type 1 binds to sensory ganglion neurons', Annals of Neurology, vol. 34, no. 6, pp. 855-863.
Apostolski S, McAlarney T, Quattrini A, Levison SW, Rosoklija G, Lugaressi A et al. The gp120 glycoprotein of human immunodeficiency virus type 1 binds to sensory ganglion neurons. Annals of Neurology. 1993 Dec;34(6):855-863.
Apostolski, Slobodan ; McAlarney, Terence ; Quattrini, Angelo ; Levison, Steven W. ; Rosoklija, Gorazd ; Lugaressi, Alessandra ; Corbo, Massimo ; Sadiq, Saud A. ; Lederman, Seth ; Hays, Arthur P. ; Latov, Norman. / The gp120 glycoprotein of human immunodeficiency virus type 1 binds to sensory ganglion neurons. In: Annals of Neurology. 1993 ; Vol. 34, No. 6. pp. 855-863.
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