The Gsα gene: Predominant maternal origin of transcription in human thyroid gland and gonads

Giovanna Mantovani, Emilia Ballare, Enza Giammona, Paolo Beck-Peccoz, Anna Spada

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Mutations in the guanine nucleotide binding α-subunit 1 gene (GNAS1) cause Albright's hereditary osteodistrophy, and the parent of transmission determines variable phenotypic expression of the disease. This has suggested that GNAS1 may be under tissue-specific imprinting control, although studies so far available have failed to clearly define the pattern of GNAS1 expression in humans. To establish if GNAS1 is imprinted in human endocrine tissues, we selected 14 thyroid, 10 granulosa cell, 13 pituitary (3 normal glands, 7 GH-secreting adenomas, and 3 nonfunctioning adenomas), 3 adrenal, and 11 lymphocyte samples shown to be heterozygous for a known polymorphism in exon 5. RNA from these tissues was analyzed by RT-PCR, and expression from both parental alleles was evaluated by enzymatic digestion and subsequent quantification of the resulting fragments. The parental origin of Gsα was assessed by evaluating neuroendocrine secretory protein 55 and extra large αs-like protein transcripts, which have been shown to be monoallelically and parent-specifically expressed from the maternal and paternal allele, respectively. By this approach, the great majority of thyroid (n = 12), ovarian (n = 7), and pituitary (n = 11) samples showed an almost exclusive or significantly predominant expression of the maternal allele over the paternal one, whereas in lymphocyte and adrenal samples both alleles were equally expressed. Our results provide evidence for a predominant maternal origin of GNAS1 transcripts in different human adult endocrine tissues, particularly thyroid, ovary, and pituitary, and strongly suggest that this mechanism may play a crucial role in the determination of the phenotypic expression of Albright's hereditary osteodistrophy.

Original languageEnglish
Pages (from-to)4736-4740
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume87
Issue number10
DOIs
Publication statusPublished - Oct 1 2002

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Gonads
Transcription
Thyroid Gland
Genes
Alleles
Mothers
Tissue
Lymphocytes
Adenoma
Guanine Nucleotides
Granulosa Cells
Polymorphism
Digestion
Ovary
Exons
Proteins
RNA
Polymerase Chain Reaction
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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The Gsα gene : Predominant maternal origin of transcription in human thyroid gland and gonads. / Mantovani, Giovanna; Ballare, Emilia; Giammona, Enza; Beck-Peccoz, Paolo; Spada, Anna.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 87, No. 10, 01.10.2002, p. 4736-4740.

Research output: Contribution to journalArticle

Mantovani, Giovanna ; Ballare, Emilia ; Giammona, Enza ; Beck-Peccoz, Paolo ; Spada, Anna. / The Gsα gene : Predominant maternal origin of transcription in human thyroid gland and gonads. In: Journal of Clinical Endocrinology and Metabolism. 2002 ; Vol. 87, No. 10. pp. 4736-4740.
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