The heat shock response in xenopus oocytes, embryos, and somatic cells: A regulatory role for chromatin

N. Landsberger, M. Ranjan, G. Almouzni, D. Stump, A. P. Wolffe

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The heat shock response in Xenopus laevis has been reported to be developmentally regulated at the transcriptional level. We find that the heat shock response of an exogenous Xenopus hsp70 gene introduced into Xenopus oocytes, embryos, and somatic cells is dependent on the transcriptional assay conditions employed. Under conditions of efficient chromatin assembly, transcription from the Xenopus hsp70 gene promoter is repressed in oocytes and embryos, yet the promoter responds to heat shock by activating transcription. Under conditions of inefficient chromatin assembly, the Xenopus hsp70 gene is constitutively active in oocytes and somatic cells. Our results resolve previous controversy concerning the existence of a heat shock response for the hsp70 promoter in oocytes and illustrate the importance of considering chromatin assembly as a contributory factor in reconstructing the developmental control of gene expression.

Original languageEnglish
Pages (from-to)62-74
Number of pages13
JournalDevelopmental Biology
Volume170
Issue number1
DOIs
Publication statusPublished - 1995

Fingerprint

Heat-Shock Response
Xenopus
Chromatin
Oocytes
Chromatin Assembly and Disassembly
Embryonic Structures
Genes
Developmental Genes
Xenopus laevis
Shock
Hot Temperature
Gene Expression

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Cite this

The heat shock response in xenopus oocytes, embryos, and somatic cells : A regulatory role for chromatin. / Landsberger, N.; Ranjan, M.; Almouzni, G.; Stump, D.; Wolffe, A. P.

In: Developmental Biology, Vol. 170, No. 1, 1995, p. 62-74.

Research output: Contribution to journalArticle

Landsberger, N. ; Ranjan, M. ; Almouzni, G. ; Stump, D. ; Wolffe, A. P. / The heat shock response in xenopus oocytes, embryos, and somatic cells : A regulatory role for chromatin. In: Developmental Biology. 1995 ; Vol. 170, No. 1. pp. 62-74.
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