The hepatitis B virus (HBV) precore protein inhibits HBV replication in transgenic mice?

Luca G. Guidotti, Brent Matzke, Claudio Pasquinelli, Jan M. Shoenberger, Charles E. Rogler, Francis V. Chisari

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we examined the ability of the hepatitis B virus (HBV) precore, envelope, and X gene products to modulate HBV replication in the livers of transgenic mice that replicate the virus. Hepatic HBV replication was not affected by overexpression of the envelope or X gene products when these animals were crossed with transgenic mice that express the corresponding viral genes in the hepatocyte. Overexpression of the precore protein, however, eliminated nucleocapsid particles from the cytoplasm of the hepatocytes and abolished HBV replication without affecting the hepatic steady-state content of pregenomic HBV RNA. These observations suggest that the precore protein can exert a dominant negative effect on HBV replication, presumably at the level of nucleocapsid particle maturation or stability, suggesting an important role for this enigmatic vital protein in the HBV life cycle.

Original languageEnglish
Pages (from-to)7056-7061
Number of pages6
JournalJournal of Virology
Volume70
Issue number10
Publication statusPublished - Oct 1996

ASJC Scopus subject areas

  • Immunology

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