The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements

Maria Laura Avantaggiati, Gioacchino Natoli, Clara Balsano, Paolo Chirillo, Marco Artini, Elisabetta De Marzio, Daniela Collepardo, Massimo Levrero

Research output: Contribution to journalArticle

Abstract

The hepatitis B virus (HBV) X protein (pX) stimulates transcription regulated by cis-acting elements that control many viral and cellular genes, including the c-myc and the c-fos proto-oncogenes. Using several c-fos promoter deletion mutants, we found the serum-responsive element (SRE) located at - 315, the modified TPA-responsive element located at -296 (fos-AP-1 binding site, FAP) and the region spanning from nucleotide - 220 to - 120, which contains an NF1-like site and several stretches of sequence homologous to the AP-2 consensus binding sites, to be responsive to pX. pX does not modify the pattern of the retarded complexes bound to the SRE/FAP region which, in our system, appears to be occupied by SRE-binding factors. The activation of the SRE does not involve complex formation between SRE-binding factors and pX, it is not associated with an increase in serum response factor binding to the SRE and it does not determine changes in SRE mobility-shift pattern.

Original languageEnglish
Pages (from-to)1567-1574
Number of pages8
JournalOncogene
Volume8
Issue number6
Publication statusPublished - Jun 1993

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Hepatitis B virus
Serum
Binding Sites
Serum Response Factor
fos Genes
Viral Genes
Transcription Factor AP-1
Sequence Homology
Nucleotides

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Avantaggiati, M. L., Natoli, G., Balsano, C., Chirillo, P., Artini, M., De Marzio, E., ... Levrero, M. (1993). The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements. Oncogene, 8(6), 1567-1574.

The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements. / Avantaggiati, Maria Laura; Natoli, Gioacchino; Balsano, Clara; Chirillo, Paolo; Artini, Marco; De Marzio, Elisabetta; Collepardo, Daniela; Levrero, Massimo.

In: Oncogene, Vol. 8, No. 6, 06.1993, p. 1567-1574.

Research output: Contribution to journalArticle

Avantaggiati, ML, Natoli, G, Balsano, C, Chirillo, P, Artini, M, De Marzio, E, Collepardo, D & Levrero, M 1993, 'The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements', Oncogene, vol. 8, no. 6, pp. 1567-1574.
Avantaggiati ML, Natoli G, Balsano C, Chirillo P, Artini M, De Marzio E et al. The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements. Oncogene. 1993 Jun;8(6):1567-1574.
Avantaggiati, Maria Laura ; Natoli, Gioacchino ; Balsano, Clara ; Chirillo, Paolo ; Artini, Marco ; De Marzio, Elisabetta ; Collepardo, Daniela ; Levrero, Massimo. / The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements. In: Oncogene. 1993 ; Vol. 8, No. 6. pp. 1567-1574.
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