Abstract
The hepatitis B virus (HBV) X protein (pX) stimulates transcription regulated by cis-acting elements that control many viral and cellular genes, including the c-myc and the c-fos proto-oncogenes. Using several c-fos promoter deletion mutants, we found the serum-responsive element (SRE) located at - 315, the modified TPA-responsive element located at -296 (fos-AP-1 binding site, FAP) and the region spanning from nucleotide - 220 to - 120, which contains an NF1-like site and several stretches of sequence homologous to the AP-2 consensus binding sites, to be responsive to pX. pX does not modify the pattern of the retarded complexes bound to the SRE/FAP region which, in our system, appears to be occupied by SRE-binding factors. The activation of the SRE does not involve complex formation between SRE-binding factors and pX, it is not associated with an increase in serum response factor binding to the SRE and it does not determine changes in SRE mobility-shift pattern.
| Original language | English |
|---|---|
| Pages (from-to) | 1567-1574 |
| Number of pages | 8 |
| Journal | Oncogene |
| Volume | 8 |
| Issue number | 6 |
| Publication status | Published - Jun 1993 |
Fingerprint
ASJC Scopus subject areas
- Cancer Research
- Genetics
- Molecular Biology
Cite this
The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements. / Avantaggiati, Maria Laura; Natoli, Gioacchino; Balsano, Clara; Chirillo, Paolo; Artini, Marco; De Marzio, Elisabetta; Collepardo, Daniela; Levrero, Massimo.
In: Oncogene, Vol. 8, No. 6, 06.1993, p. 1567-1574.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements
AU - Avantaggiati, Maria Laura
AU - Natoli, Gioacchino
AU - Balsano, Clara
AU - Chirillo, Paolo
AU - Artini, Marco
AU - De Marzio, Elisabetta
AU - Collepardo, Daniela
AU - Levrero, Massimo
PY - 1993/6
Y1 - 1993/6
N2 - The hepatitis B virus (HBV) X protein (pX) stimulates transcription regulated by cis-acting elements that control many viral and cellular genes, including the c-myc and the c-fos proto-oncogenes. Using several c-fos promoter deletion mutants, we found the serum-responsive element (SRE) located at - 315, the modified TPA-responsive element located at -296 (fos-AP-1 binding site, FAP) and the region spanning from nucleotide - 220 to - 120, which contains an NF1-like site and several stretches of sequence homologous to the AP-2 consensus binding sites, to be responsive to pX. pX does not modify the pattern of the retarded complexes bound to the SRE/FAP region which, in our system, appears to be occupied by SRE-binding factors. The activation of the SRE does not involve complex formation between SRE-binding factors and pX, it is not associated with an increase in serum response factor binding to the SRE and it does not determine changes in SRE mobility-shift pattern.
AB - The hepatitis B virus (HBV) X protein (pX) stimulates transcription regulated by cis-acting elements that control many viral and cellular genes, including the c-myc and the c-fos proto-oncogenes. Using several c-fos promoter deletion mutants, we found the serum-responsive element (SRE) located at - 315, the modified TPA-responsive element located at -296 (fos-AP-1 binding site, FAP) and the region spanning from nucleotide - 220 to - 120, which contains an NF1-like site and several stretches of sequence homologous to the AP-2 consensus binding sites, to be responsive to pX. pX does not modify the pattern of the retarded complexes bound to the SRE/FAP region which, in our system, appears to be occupied by SRE-binding factors. The activation of the SRE does not involve complex formation between SRE-binding factors and pX, it is not associated with an increase in serum response factor binding to the SRE and it does not determine changes in SRE mobility-shift pattern.
UR - http://www.scopus.com/inward/record.url?scp=0027310496&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027310496&partnerID=8YFLogxK
M3 - Article
C2 - 8502480
AN - SCOPUS:0027310496
VL - 8
SP - 1567
EP - 1574
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 6
ER -