Abstract
It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.
| Original language | English |
|---|---|
| Pages (from-to) | 63-71 |
| Number of pages | 9 |
| Journal | Archives of virology. Supplementum |
| Volume | 8 |
| Publication status | Published - 1993 |
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ASJC Scopus subject areas
- Immunology and Microbiology(all)
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The hepatitis B virus X gene product transactivates the HIV-LTR in vivo. / Balsano, C.; Billet, O.; Bennoun, M.; Cavard, C.; Zider, A.; Grimber, G.; Natoli, G.; Briand, P.; Levrero, M.
In: Archives of virology. Supplementum, Vol. 8, 1993, p. 63-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.
AU - Balsano, C.
AU - Billet, O.
AU - Bennoun, M.
AU - Cavard, C.
AU - Zider, A.
AU - Grimber, G.
AU - Natoli, G.
AU - Briand, P.
AU - Levrero, M.
PY - 1993
Y1 - 1993
N2 - It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.
AB - It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.
UR - http://www.scopus.com/inward/record.url?scp=0027711871&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027711871&partnerID=8YFLogxK
M3 - Article
C2 - 8260878
AN - SCOPUS:0027711871
VL - 8
SP - 63
EP - 71
JO - Archives of Virology, Supplement
JF - Archives of Virology, Supplement
SN - 0939-1983
ER -