The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.

C. Balsano, O. Billet, M. Bennoun, C. Cavard, A. Zider, G. Grimber, G. Natoli, P. Briand, M. Levrero

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.

Original languageEnglish
Pages (from-to)63-71
Number of pages9
JournalArchives of virology. Supplementum
Volume8
Publication statusPublished - 1993

Fingerprint

HIV Long Terminal Repeat
Hepatitis B virus
Transgenic Mice
HIV-1
Genetic Hybridization
Lac Operon
Antithrombin III
Chloramphenicol
Regulator Genes
Transferases
Reporter Genes
Immunoprecipitation
Open Reading Frames
Genes
Proteins
Viruses
Liver

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Balsano, C., Billet, O., Bennoun, M., Cavard, C., Zider, A., Grimber, G., ... Levrero, M. (1993). The hepatitis B virus X gene product transactivates the HIV-LTR in vivo. Archives of virology. Supplementum, 8, 63-71.

The hepatitis B virus X gene product transactivates the HIV-LTR in vivo. / Balsano, C.; Billet, O.; Bennoun, M.; Cavard, C.; Zider, A.; Grimber, G.; Natoli, G.; Briand, P.; Levrero, M.

In: Archives of virology. Supplementum, Vol. 8, 1993, p. 63-71.

Research output: Contribution to journalArticle

Balsano, C, Billet, O, Bennoun, M, Cavard, C, Zider, A, Grimber, G, Natoli, G, Briand, P & Levrero, M 1993, 'The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.', Archives of virology. Supplementum, vol. 8, pp. 63-71.
Balsano C, Billet O, Bennoun M, Cavard C, Zider A, Grimber G et al. The hepatitis B virus X gene product transactivates the HIV-LTR in vivo. Archives of virology. Supplementum. 1993;8:63-71.
Balsano, C. ; Billet, O. ; Bennoun, M. ; Cavard, C. ; Zider, A. ; Grimber, G. ; Natoli, G. ; Briand, P. ; Levrero, M. / The hepatitis B virus X gene product transactivates the HIV-LTR in vivo. In: Archives of virology. Supplementum. 1993 ; Vol. 8. pp. 63-71.
@article{c5870883bed94590b274e55a7203f62f,
title = "The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.",
abstract = "It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.",
author = "C. Balsano and O. Billet and M. Bennoun and C. Cavard and A. Zider and G. Grimber and G. Natoli and P. Briand and M. Levrero",
year = "1993",
language = "English",
volume = "8",
pages = "63--71",
journal = "Archives of Virology, Supplement",
issn = "0939-1983",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.

AU - Balsano, C.

AU - Billet, O.

AU - Bennoun, M.

AU - Cavard, C.

AU - Zider, A.

AU - Grimber, G.

AU - Natoli, G.

AU - Briand, P.

AU - Levrero, M.

PY - 1993

Y1 - 1993

N2 - It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.

AB - It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.

UR - http://www.scopus.com/inward/record.url?scp=0027711871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027711871&partnerID=8YFLogxK

M3 - Article

C2 - 8260878

AN - SCOPUS:0027711871

VL - 8

SP - 63

EP - 71

JO - Archives of Virology, Supplement

JF - Archives of Virology, Supplement

SN - 0939-1983

ER -