The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.

C. Balsano; O. Billet; M. Bennoun; C. Cavard; A. Zider; G. Grimber; G. Natoli; P. Briand; M. Levrero

The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.

C. Balsano, O. Billet, M. Bennoun, C. Cavard, A. Zider, G. Grimber, G. Natoli, P. Briand, M. Levrero

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article › peer-review

Abstract

It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.

Original language	English
Pages (from-to)	63-71
Number of pages	9
Journal	Archives of virology. Supplementum
Volume	8
Publication status	Published - 1993

ASJC Scopus subject areas

Immunology and Microbiology(all)

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title = "The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.",

abstract = "It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.",

author = "C. Balsano and O. Billet and M. Bennoun and C. Cavard and A. Zider and G. Grimber and G. Natoli and P. Briand and M. Levrero",

year = "1993",

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journal = "Archives of Virology, Supplement",

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T1 - The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.

AU - Balsano, C.

AU - Billet, O.

AU - Bennoun, M.

AU - Cavard, C.

AU - Zider, A.

AU - Grimber, G.

AU - Natoli, G.

AU - Briand, P.

AU - Levrero, M.

PY - 1993

Y1 - 1993

N2 - It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.

AB - It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.

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