The Histone H3 Lysine-27 Demethylase Jmjd3 Links Inflammation to Inhibition of Polycomb-Mediated Gene Silencing

Francesca De Santa, Maria Grazia Totaro, Elena Prosperini, Samuele Notarbartolo, Giuseppe Testa, Gioacchino Natoli

Research output: Contribution to journalArticle

Abstract

Epigenetic chromatin marks restrict the ability of differentiated cells to change gene expression programs in response to environmental cues and to transdifferentiate. Polycomb group (PcG) proteins mediate gene silencing and repress transdifferentiation in a manner dependent on histone H3 lysine 27 trimethylation (H3K27me3). However, macrophages migrated into inflamed tissues can transdifferentiate, but it is unknown whether inflammation alters PcG-dependent silencing. Here we show that the JmjC-domain protein Jmjd3 is a H3K27me demethylase expressed in macrophages in response to bacterial products and inflammatory cytokines. Jmjd3 binds PcG target genes and regulates their H3K27me3 levels and transcriptional activity. The discovery of an inducible enzyme that erases a histone mark controlling differentiation and cell identity provides a link between inflammation and reprogramming of the epigenome, which could be the basis for macrophage plasticity and might explain the differentiation abnormalities in chronic inflammation.

Original languageEnglish
Pages (from-to)1083-1094
Number of pages12
JournalCell
Volume130
Issue number6
DOIs
Publication statusPublished - Sep 21 2007

Fingerprint

Macrophages
Gene Silencing
Histones
Lysine
Genes
Inflammation
Histone Code
Polycomb-Group Proteins
Aptitude
Epigenomics
Gene expression
Chromatin
Cues
Plasticity
Cell Differentiation
Tissue
Cytokines
Gene Expression
Enzymes
Inhibition (Psychology)

Keywords

  • HUMDISEASE
  • PROTEINS
  • SIGNALING

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The Histone H3 Lysine-27 Demethylase Jmjd3 Links Inflammation to Inhibition of Polycomb-Mediated Gene Silencing. / De Santa, Francesca; Totaro, Maria Grazia; Prosperini, Elena; Notarbartolo, Samuele; Testa, Giuseppe; Natoli, Gioacchino.

In: Cell, Vol. 130, No. 6, 21.09.2007, p. 1083-1094.

Research output: Contribution to journalArticle

De Santa, Francesca ; Totaro, Maria Grazia ; Prosperini, Elena ; Notarbartolo, Samuele ; Testa, Giuseppe ; Natoli, Gioacchino. / The Histone H3 Lysine-27 Demethylase Jmjd3 Links Inflammation to Inhibition of Polycomb-Mediated Gene Silencing. In: Cell. 2007 ; Vol. 130, No. 6. pp. 1083-1094.
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