The HIV-1 tat protein modulates CD4 expression in human T cells through the induction of miR-222

Elisa Orecchini, Margherita Doria, Alessandro Michienzi, Erica Giuliani, Lia Vassena, Silvia Anna Ciafrè, Maria Giulia Farace, Silvia Galardi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Several cellular microRNAs show substantial changes in expression during HIV-1 infection and their active role in the viral life cycle is progressively emerging. In the present study, we found that HIV-1 infection of Jurkat T cells significantly induces the expression of miR-222. We show that this induction depends on HIV-1 Tat protein, which is able to increase the transcriptional activity of NFkB on miR-222 promoter. Moreover, we demonstrate that miR-222 directly targets CD4, a key receptor for HIV-1, thus reducing its expression. We propose that Tat, by inducing miR-222 expression, complements the CD4 downregulation activity exerted by other viral proteins (i.e., Nef, Vpu, and Env), and we suggest that this represents a novel mechanism through which HIV-1 efficiently represses CD4 expression in infected cells.

Original languageEnglish
Pages (from-to)334-338
Number of pages5
JournalRNA Biology
Volume11
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Human Immunodeficiency Virus tat Gene Products
HIV-1
T-Lymphocytes
HIV Infections
Jurkat Cells
Viral Proteins
Life Cycle Stages
MicroRNAs
Down-Regulation

Keywords

  • CD4
  • HIV-1
  • MicroRNAs
  • NFkB
  • Post-transcriptional regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

The HIV-1 tat protein modulates CD4 expression in human T cells through the induction of miR-222. / Orecchini, Elisa; Doria, Margherita; Michienzi, Alessandro; Giuliani, Erica; Vassena, Lia; Ciafrè, Silvia Anna; Farace, Maria Giulia; Galardi, Silvia.

In: RNA Biology, Vol. 11, No. 4, 2014, p. 334-338.

Research output: Contribution to journalArticle

Orecchini, E, Doria, M, Michienzi, A, Giuliani, E, Vassena, L, Ciafrè, SA, Farace, MG & Galardi, S 2014, 'The HIV-1 tat protein modulates CD4 expression in human T cells through the induction of miR-222', RNA Biology, vol. 11, no. 4, pp. 334-338. https://doi.org/10.4161/rna.28372
Orecchini, Elisa ; Doria, Margherita ; Michienzi, Alessandro ; Giuliani, Erica ; Vassena, Lia ; Ciafrè, Silvia Anna ; Farace, Maria Giulia ; Galardi, Silvia. / The HIV-1 tat protein modulates CD4 expression in human T cells through the induction of miR-222. In: RNA Biology. 2014 ; Vol. 11, No. 4. pp. 334-338.
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