The HMGA1 pseudogene 7 induces miR-483 and miR-675 upregulation by activating Egr1 through a ceRNA mechanism

Marco De Martino, Giuseppe Palma, Amalia Azzariti, Claudio Arra, Alfredo Fusco, Francesco Esposito

Research output: Contribution to journalArticle

Abstract

Several studies have established that pseudogene mRNAs can work as competing endogenous RNAs and, when deregulated, play a key role in the onset of human neoplasias. Recently, we have isolated two HMGA1 pseudogenes, HMGA1P6 and HMGA1P7. These pseudoenes have a critical role in cancer progression, acting as micro RNA (miRNA) sponges for HMGA1 and other cancer-related genes. HMGA1 pseudogenes were found overexpressed in several human carcinomas, and their expression levels positively correlate with an advanced cancer stage and a poor prognosis. In order to investigate the molecular alterations following HMGA1 pseudogene 7 overexpression, we carried out miRNA sequencing analysis on HMGA1P7 overexpressing mouse embryonic fibroblasts. Intriguingly, the most upregulated miRNAs were miR-483 and miR-675 that have been described as key regulators in cancer progression. Here, we report that HMGA1P7 upregulates miR-483 and miR-675 through a competing endogenous RNA mechanism with Egr1, a transcriptional factor that positively regulates miR-483 and miR-675 expression.

Original languageEnglish
Article number330
JournalGenes
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 17 2017

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Keywords

  • CeRNA
  • HMGA1 pseudogenes
  • HMGA1P7
  • Micro RNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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