The HTL1 gene (YCR020W-b) of Saccharomyces cerevisiae is necessary for growth at 37°C, and for the conservation of chromosome stability and fertility

Chiara Lanzuolo; Sara Ederle; Alessandra Pollice; Francesco Russo; Aurora Storlazzi; John F. Pulitzer

doi:10.1002/yea.778

The HTL1 gene (YCR020W-b) of Saccharomyces cerevisiae is necessary for growth at 37°C, and for the conservation of chromosome stability and fertility

Chiara Lanzuolo, Sara Ederle, Alessandra Pollice, Francesco Russo, Aurora Storlazzi, John F. Pulitzer

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

A small 78 codon ORF, named HTL1 (Chen et al., unpublished results), situated between loci MAK31 and HSP30 on chromosome III of Saccharomyces cerevisiae, is required for growth at 37°C. In this communication, we characterize the ORF and show that disruption of HTL1, besides preventing growth at 37°C, causes genetic and/or epigenetic instability at 26°C: ploidy increases in about 10% of cells grown from individual disruptants and a fraction of disruptant clones are predestined to a rapid and progressive loss of fertility during growth at 26°C.

Original language	English
Pages (from-to)	1317-1330
Number of pages	14
Journal	Yeast
Volume	18
Issue number	14
DOIs	https://doi.org/10.1002/yea.778
Publication status	Published - 2001

Keywords

Chromosome segregation
Electroporation
Genome stability
Ploidy
Saccharomyces cerevisiae HTL1
Thermolability
Yeast

ASJC Scopus subject areas

Agricultural and Biological Sciences (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
Biochemistry
Biotechnology
Bioengineering
Microbiology

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10.1002/yea.778

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abstract = "A small 78 codon ORF, named HTL1 (Chen et al., unpublished results), situated between loci MAK31 and HSP30 on chromosome III of Saccharomyces cerevisiae, is required for growth at 37°C. In this communication, we characterize the ORF and show that disruption of HTL1, besides preventing growth at 37°C, causes genetic and/or epigenetic instability at 26°C: ploidy increases in about 10% of cells grown from individual disruptants and a fraction of disruptant clones are predestined to a rapid and progressive loss of fertility during growth at 26°C.",

keywords = "Chromosome segregation, Electroporation, Genome stability, Ploidy, Saccharomyces cerevisiae HTL1, Thermolability, Yeast",

author = "Chiara Lanzuolo and Sara Ederle and Alessandra Pollice and Francesco Russo and Aurora Storlazzi and Pulitzer, {John F.}",

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AU - Lanzuolo, Chiara

AU - Ederle, Sara

AU - Pollice, Alessandra

AU - Russo, Francesco

AU - Storlazzi, Aurora

AU - Pulitzer, John F.

PY - 2001

Y1 - 2001

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AB - A small 78 codon ORF, named HTL1 (Chen et al., unpublished results), situated between loci MAK31 and HSP30 on chromosome III of Saccharomyces cerevisiae, is required for growth at 37°C. In this communication, we characterize the ORF and show that disruption of HTL1, besides preventing growth at 37°C, causes genetic and/or epigenetic instability at 26°C: ploidy increases in about 10% of cells grown from individual disruptants and a fraction of disruptant clones are predestined to a rapid and progressive loss of fertility during growth at 26°C.

KW - Chromosome segregation

KW - Electroporation

KW - Genome stability

KW - Ploidy

KW - Saccharomyces cerevisiae HTL1

KW - Thermolability

KW - Yeast

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The HTL1 gene (YCR020W-b) of Saccharomyces cerevisiae is necessary for growth at 37°C, and for the conservation of chromosome stability and fertility

Abstract

Keywords

ASJC Scopus subject areas

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