Abstract
A small 78 codon ORF, named HTL1 (Chen et al., unpublished results), situated between loci MAK31 and HSP30 on chromosome III of Saccharomyces cerevisiae, is required for growth at 37°C. In this communication, we characterize the ORF and show that disruption of HTL1, besides preventing growth at 37°C, causes genetic and/or epigenetic instability at 26°C: ploidy increases in about 10% of cells grown from individual disruptants and a fraction of disruptant clones are predestined to a rapid and progressive loss of fertility during growth at 26°C.
Original language | English |
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Pages (from-to) | 1317-1330 |
Number of pages | 14 |
Journal | Yeast |
Volume | 18 |
Issue number | 14 |
DOIs | |
Publication status | Published - 2001 |
Keywords
- Chromosome segregation
- Electroporation
- Genome stability
- Ploidy
- Saccharomyces cerevisiae HTL1
- Thermolability
- Yeast
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Bioengineering
- Microbiology