The HTL1 gene (YCR020W-b) of Saccharomyces cerevisiae is necessary for growth at 37°C, and for the conservation of chromosome stability and fertility

Chiara Lanzuolo, Sara Ederle, Alessandra Pollice, Francesco Russo, Aurora Storlazzi, John F. Pulitzer

Research output: Contribution to journalArticlepeer-review

Abstract

A small 78 codon ORF, named HTL1 (Chen et al., unpublished results), situated between loci MAK31 and HSP30 on chromosome III of Saccharomyces cerevisiae, is required for growth at 37°C. In this communication, we characterize the ORF and show that disruption of HTL1, besides preventing growth at 37°C, causes genetic and/or epigenetic instability at 26°C: ploidy increases in about 10% of cells grown from individual disruptants and a fraction of disruptant clones are predestined to a rapid and progressive loss of fertility during growth at 26°C.

Original languageEnglish
Pages (from-to)1317-1330
Number of pages14
JournalYeast
Volume18
Issue number14
DOIs
Publication statusPublished - 2001

Keywords

  • Chromosome segregation
  • Electroporation
  • Genome stability
  • Ploidy
  • Saccharomyces cerevisiae HTL1
  • Thermolability
  • Yeast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

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