The HtrA1 serine protease is down-regulated during human melanoma progression and represses growth of metastatic melanoma cells

Alfonso Baldi, Antonio De Luca, Monica Morini, Tullio Battista, Armando Felsani, Feliciano Baldi, Caterina Catricalà, Ada Amantea, Douglas M. Noonan, Adriana Albini, Pier Giorgio Natali, Daniela Lombardi, Marco G. Paggi

Research output: Contribution to journalArticle

Abstract

Differential gene expression of cell lines derived from a malignant melanoma or its autologous lymph node metastasis using cDNA arrays indicated down-regulation of PRSS11, a gene encoding the serine protease HtrA1, a homolog of the Escherichia coli protease HtrA, in the metastatic line. Stable PRSS11 overexpression in the metastatic cell line strongly inhibited proliferation, chemoinvasion and Nm23-H1 protein expression in vitro, as well as cell growth in vivo in nu/nu mice. A polyclonal anti-HtrA1 serum demonstrated a significantly higher expression in primary melanomas when compared to unrelated metastatic lesions in a human melanoma tissue array, and down-modulation of HtrA1 expression in autologous lymph node melanoma metastases in seven out of 11 cases examined. These results suggest that down-regulation of PRSS11 and HtrA1 expression may represent an indicator of melanoma progression.

Original languageEnglish
Pages (from-to)6684-6688
Number of pages5
JournalOncogene
Volume21
Issue number43
DOIs
Publication statusPublished - Sep 26 2002

Keywords

  • HtrA1
  • Melanoma
  • PRSS11
  • Serine protease
  • Tumor progression

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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  • Cite this

    Baldi, A., De Luca, A., Morini, M., Battista, T., Felsani, A., Baldi, F., Catricalà, C., Amantea, A., Noonan, D. M., Albini, A., Natali, P. G., Lombardi, D., & Paggi, M. G. (2002). The HtrA1 serine protease is down-regulated during human melanoma progression and represses growth of metastatic melanoma cells. Oncogene, 21(43), 6684-6688. https://doi.org/10.1038/sj.onc.1205911