α-1-antitrypsin (α-1AT) present in large amounts in human serum and. synthesized predominantly in hepatocytes, is the most abundant protease inhibitor and α-1AT mutant proteins are associated with heriditary disorders. To investigate the regulation of the normal human α-1AT gene, we have microinjected fertilized mouse eggs with a 17.5 kb DNA fragment containing the entire gene with 7 kb 5′ and 0.3 kb 3′ flanking sequences. We show that this DNA fragment contains all the information for efficient, accurate and tissue-specific expression. High serum concentration of the human protein was found in three independent transgenic mouse lines. The human α-1AT RNA is transcribed efficiently in liver, kidney and macrophages and we demonstrate that two different promoters are used for the expression in liver and macrophages of the transgenic mice.
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis