The human astrocytoma cell line U373MG produces monocyte chemotactic protein (MCP)-1 upon stimulation with β-amyloid protein

Elisabetta Prat, Pierluigi Baron, Lucia Meda, Elio Scarpini, Daniela Galimberti, Gianluca Ardolino, Anna Catania, Guglielmo Scarlato

Research output: Contribution to journalArticle

Abstract

Astrocytes associated with β-amyloid (Aβ) accumulate in senile plaques of Alzheimer's disease (AD). To investigate the biological effects of Aβ/astrocyte interaction, we examined chemokine production by the human astrocytoma cell line U373MG stimulated with Aβ peptides. Northern blot analysis and specific immunoassays demonstrate that Aβ [1-42] and Aβ [25-35] induce mRNA expression and release of monocyte chemotactic protein (MCP)-1 but not of γ-interferon inducible protein (IP)-10 by U373MG cells. The observation that Aβ induces astrocyte production of the potent microglia chemoattractant MCP-1 contributes to understanding mechanism of damage exerted by Aβ in AD senile plaques. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)177-180
Number of pages4
JournalNeuroscience Letters
Volume283
Issue number3
DOIs
Publication statusPublished - Apr 14 2000

Keywords

  • β-amyloid
  • γ-Interferon inducible protein (IP)-10
  • Alzheimer's disease
  • Astrocytes
  • Chemokines
  • Monocyte chemotactic protein (MCP)-1

ASJC Scopus subject areas

  • Neuroscience(all)

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