The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments

Maria Isabel Fernandez, Mélina L. Heuzé, Carolina Martinez-Cingolani, Elisabetta Volpe, Marie Helene Donnadieu, Matthieu Piel, Bernhard Homey, Ana Maria Lennon-Duménil, Vassili Soumelis

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) need to migrate in the interstitial environment of peripheral tissues to reach secondary lymphoid organs and initiate a suitable immune response. Whether and how inflamed tissues instruct DCs to emigrate is not fully understood. In this study, we report the unexpected finding that the epithelial-derived cytokine TSLP triggers chemokinesis of resting primary human DCs in a cell-autonomous manner. TSLP induced the polarization of both microtubule and actin cytoskeletons and promoted DC 3-dimensional migration in transwell as well as in microfabricated channels that mimic the confined environment of peripheral tissues. TSLP-induced migration relied on the actin-based motor myosin II and was inhibited by blebbistatin. Accordingly, TSLP triggered the redistribution of phosphorylated myosin II regulatory light chain to the actin cortex, indicating that TSLP induces DC migration by promoting actomyosin contractility. Thus, TSLP produced by epithelial cells in inflamed tissue has a critical function in licensing DCs for cell-autonomous migration. This indicates that cytokines can directly trigger cell migration, which has important implications in immune physiopathology and vaccine design.

Original languageEnglish
Pages (from-to)3862-3869
Number of pages8
JournalBlood
Volume118
Issue number14
DOIs
Publication statusPublished - Oct 6 2011

Fingerprint

Dendritic Cells
Cell Movement
Tissue
Myosin Type II
Actins
Actomyosin
Myosin Light Chains
Licensure
Actin Cytoskeleton
Microtubules
thymic stromal lymphopoietin
Vaccines
Epithelial Cells
Polarization
Cytokines

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Fernandez, M. I., Heuzé, M. L., Martinez-Cingolani, C., Volpe, E., Donnadieu, M. H., Piel, M., ... Soumelis, V. (2011). The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments. Blood, 118(14), 3862-3869. https://doi.org/10.1182/blood-2010-12-323089

The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments. / Fernandez, Maria Isabel; Heuzé, Mélina L.; Martinez-Cingolani, Carolina; Volpe, Elisabetta; Donnadieu, Marie Helene; Piel, Matthieu; Homey, Bernhard; Lennon-Duménil, Ana Maria; Soumelis, Vassili.

In: Blood, Vol. 118, No. 14, 06.10.2011, p. 3862-3869.

Research output: Contribution to journalArticle

Fernandez, MI, Heuzé, ML, Martinez-Cingolani, C, Volpe, E, Donnadieu, MH, Piel, M, Homey, B, Lennon-Duménil, AM & Soumelis, V 2011, 'The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments', Blood, vol. 118, no. 14, pp. 3862-3869. https://doi.org/10.1182/blood-2010-12-323089
Fernandez MI, Heuzé ML, Martinez-Cingolani C, Volpe E, Donnadieu MH, Piel M et al. The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments. Blood. 2011 Oct 6;118(14):3862-3869. https://doi.org/10.1182/blood-2010-12-323089
Fernandez, Maria Isabel ; Heuzé, Mélina L. ; Martinez-Cingolani, Carolina ; Volpe, Elisabetta ; Donnadieu, Marie Helene ; Piel, Matthieu ; Homey, Bernhard ; Lennon-Duménil, Ana Maria ; Soumelis, Vassili. / The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments. In: Blood. 2011 ; Vol. 118, No. 14. pp. 3862-3869.
@article{ee078033768d49ce9c0e71bcfb853580,
title = "The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments",
abstract = "Dendritic cells (DCs) need to migrate in the interstitial environment of peripheral tissues to reach secondary lymphoid organs and initiate a suitable immune response. Whether and how inflamed tissues instruct DCs to emigrate is not fully understood. In this study, we report the unexpected finding that the epithelial-derived cytokine TSLP triggers chemokinesis of resting primary human DCs in a cell-autonomous manner. TSLP induced the polarization of both microtubule and actin cytoskeletons and promoted DC 3-dimensional migration in transwell as well as in microfabricated channels that mimic the confined environment of peripheral tissues. TSLP-induced migration relied on the actin-based motor myosin II and was inhibited by blebbistatin. Accordingly, TSLP triggered the redistribution of phosphorylated myosin II regulatory light chain to the actin cortex, indicating that TSLP induces DC migration by promoting actomyosin contractility. Thus, TSLP produced by epithelial cells in inflamed tissue has a critical function in licensing DCs for cell-autonomous migration. This indicates that cytokines can directly trigger cell migration, which has important implications in immune physiopathology and vaccine design.",
author = "Fernandez, {Maria Isabel} and Heuz{\'e}, {M{\'e}lina L.} and Carolina Martinez-Cingolani and Elisabetta Volpe and Donnadieu, {Marie Helene} and Matthieu Piel and Bernhard Homey and Lennon-Dum{\'e}nil, {Ana Maria} and Vassili Soumelis",
year = "2011",
month = "10",
day = "6",
doi = "10.1182/blood-2010-12-323089",
language = "English",
volume = "118",
pages = "3862--3869",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "14",

}

TY - JOUR

T1 - The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments

AU - Fernandez, Maria Isabel

AU - Heuzé, Mélina L.

AU - Martinez-Cingolani, Carolina

AU - Volpe, Elisabetta

AU - Donnadieu, Marie Helene

AU - Piel, Matthieu

AU - Homey, Bernhard

AU - Lennon-Duménil, Ana Maria

AU - Soumelis, Vassili

PY - 2011/10/6

Y1 - 2011/10/6

N2 - Dendritic cells (DCs) need to migrate in the interstitial environment of peripheral tissues to reach secondary lymphoid organs and initiate a suitable immune response. Whether and how inflamed tissues instruct DCs to emigrate is not fully understood. In this study, we report the unexpected finding that the epithelial-derived cytokine TSLP triggers chemokinesis of resting primary human DCs in a cell-autonomous manner. TSLP induced the polarization of both microtubule and actin cytoskeletons and promoted DC 3-dimensional migration in transwell as well as in microfabricated channels that mimic the confined environment of peripheral tissues. TSLP-induced migration relied on the actin-based motor myosin II and was inhibited by blebbistatin. Accordingly, TSLP triggered the redistribution of phosphorylated myosin II regulatory light chain to the actin cortex, indicating that TSLP induces DC migration by promoting actomyosin contractility. Thus, TSLP produced by epithelial cells in inflamed tissue has a critical function in licensing DCs for cell-autonomous migration. This indicates that cytokines can directly trigger cell migration, which has important implications in immune physiopathology and vaccine design.

AB - Dendritic cells (DCs) need to migrate in the interstitial environment of peripheral tissues to reach secondary lymphoid organs and initiate a suitable immune response. Whether and how inflamed tissues instruct DCs to emigrate is not fully understood. In this study, we report the unexpected finding that the epithelial-derived cytokine TSLP triggers chemokinesis of resting primary human DCs in a cell-autonomous manner. TSLP induced the polarization of both microtubule and actin cytoskeletons and promoted DC 3-dimensional migration in transwell as well as in microfabricated channels that mimic the confined environment of peripheral tissues. TSLP-induced migration relied on the actin-based motor myosin II and was inhibited by blebbistatin. Accordingly, TSLP triggered the redistribution of phosphorylated myosin II regulatory light chain to the actin cortex, indicating that TSLP induces DC migration by promoting actomyosin contractility. Thus, TSLP produced by epithelial cells in inflamed tissue has a critical function in licensing DCs for cell-autonomous migration. This indicates that cytokines can directly trigger cell migration, which has important implications in immune physiopathology and vaccine design.

UR - http://www.scopus.com/inward/record.url?scp=80053620974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053620974&partnerID=8YFLogxK

U2 - 10.1182/blood-2010-12-323089

DO - 10.1182/blood-2010-12-323089

M3 - Article

C2 - 21772055

AN - SCOPUS:80053620974

VL - 118

SP - 3862

EP - 3869

JO - Blood

JF - Blood

SN - 0006-4971

IS - 14

ER -