The human homolog of fission yeast Rad17 is implicated in tumor growth

Giovanni L. Beretta, Laura Gatti, Michelandrea De Cesare, Elisabetta Corna, Stella Tinelli, Nives Carenini, Franco Zunino, Paola Perego

Research output: Contribution to journalArticle

Abstract

The Schizosaccharomyces pombe rad17 is a checkpoint protein critical for maintenance of genomic stability. Since the loss of checkpoint control is a common feature of tumor cells, we investigated the biological function of the human homolog hRAD17. Expression of hRAD17 in a fission yeast rad17 deleted strain reduced growth of yeast colonies and caused slower progression through cell cycle. Immunoprecipitated hRad17 exhibited exonuclease activity. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. Our results support that hRAD17, similarly to other human genes involved in checkpoint mechanisms, plays a role in control of tumor growth.

Original languageEnglish
Pages (from-to)194-202
Number of pages9
JournalCancer Letters
Volume266
Issue number2
DOIs
Publication statusPublished - Aug 8 2008

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Keywords

  • RAD17
  • Tumor growth
  • Yeast cells

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Beretta, G. L., Gatti, L., Cesare, M. D., Corna, E., Tinelli, S., Carenini, N., Zunino, F., & Perego, P. (2008). The human homolog of fission yeast Rad17 is implicated in tumor growth. Cancer Letters, 266(2), 194-202. https://doi.org/10.1016/j.canlet.2008.02.057