The human homolog of fission yeast Rad17 is implicated in tumor growth

Giovanni L. Beretta; Laura Gatti; Michelandrea De Cesare; Elisabetta Corna; Stella Tinelli; Nives Carenini; Franco Zunino; Paola Perego

doi:10.1016/j.canlet.2008.02.057

The human homolog of fission yeast Rad17 is implicated in tumor growth

Giovanni L. Beretta, Laura Gatti, Michelandrea De Cesare, Elisabetta Corna, Stella Tinelli, Nives Carenini, Franco Zunino, Paola Perego

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

The Schizosaccharomyces pombe rad17 is a checkpoint protein critical for maintenance of genomic stability. Since the loss of checkpoint control is a common feature of tumor cells, we investigated the biological function of the human homolog hRAD17. Expression of hRAD17 in a fission yeast rad17 deleted strain reduced growth of yeast colonies and caused slower progression through cell cycle. Immunoprecipitated hRad17 exhibited exonuclease activity. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. Our results support that hRAD17, similarly to other human genes involved in checkpoint mechanisms, plays a role in control of tumor growth.

Original language	English
Pages (from-to)	194-202
Number of pages	9
Journal	Cancer Letters
Volume	266
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.02.057
Publication status	Published - Aug 8 2008

Keywords

RAD17
Tumor growth
Yeast cells

ASJC Scopus subject areas

Cancer Research
Molecular Biology
Oncology

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10.1016/j.canlet.2008.02.057

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title = "The human homolog of fission yeast Rad17 is implicated in tumor growth",

abstract = "The Schizosaccharomyces pombe rad17 is a checkpoint protein critical for maintenance of genomic stability. Since the loss of checkpoint control is a common feature of tumor cells, we investigated the biological function of the human homolog hRAD17. Expression of hRAD17 in a fission yeast rad17 deleted strain reduced growth of yeast colonies and caused slower progression through cell cycle. Immunoprecipitated hRad17 exhibited exonuclease activity. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. Our results support that hRAD17, similarly to other human genes involved in checkpoint mechanisms, plays a role in control of tumor growth.",

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AU - Beretta, Giovanni L.

AU - Gatti, Laura

AU - Cesare, Michelandrea De

AU - Corna, Elisabetta

AU - Tinelli, Stella

AU - Carenini, Nives

AU - Zunino, Franco

AU - Perego, Paola

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AB - The Schizosaccharomyces pombe rad17 is a checkpoint protein critical for maintenance of genomic stability. Since the loss of checkpoint control is a common feature of tumor cells, we investigated the biological function of the human homolog hRAD17. Expression of hRAD17 in a fission yeast rad17 deleted strain reduced growth of yeast colonies and caused slower progression through cell cycle. Immunoprecipitated hRad17 exhibited exonuclease activity. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. Our results support that hRAD17, similarly to other human genes involved in checkpoint mechanisms, plays a role in control of tumor growth.

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The human homolog of fission yeast Rad17 is implicated in tumor growth

Abstract

Keywords

ASJC Scopus subject areas

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