Abstract
Viral infection may induce the cell-surface expression of PVR (CD155) that, upon recognition by its cognate activating DNAM-1 receptor present on cytotoxic lymphocytes, may promote antiviral immune responses. Here we show that expression of the human immunodeficiency virus type 1 (HIV-1) Vpr protein in Jurkat T cells increases cell-surface and total PVR levels. Analysis of mutated Vpr variants indicated that Vpr uses the same protein surfaces, and hence probably the same mechanisms, to upregulate PVR and arrest the cell cycle in the G2 phase. Moreover, we found that PVR upregulation by Vpr relied on the ability of the protein to activate the ATR kinase that triggers the DNA damage response pathway and G2 arrest. Finally, we showed that Vpr contributes to PVR up-modulation in HIV-infected CD4+ T lymphocytes and inhibits the PVR downregulating activity of the viral Nef protein.
| Original language | English |
|---|---|
| Pages (from-to) | 2664-2669 |
| Number of pages | 6 |
| Journal | Journal of General Virology |
| Volume | 94 |
| Issue number | PART 12 |
| DOIs | |
| Publication status | Published - Dec 2013 |
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ASJC Scopus subject areas
- Virology
Cite this
The human immunodeficiency virus type 1 Vpr protein upregulates PVR via activation of the ATR-mediated DNA damage response pathway. / Vassena, Lia; Giuliani, Erica; Matusali, Giulia; Cohen, Éric A.; Doria, Margherita.
In: Journal of General Virology, Vol. 94, No. PART 12, 12.2013, p. 2664-2669.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The human immunodeficiency virus type 1 Vpr protein upregulates PVR via activation of the ATR-mediated DNA damage response pathway
AU - Vassena, Lia
AU - Giuliani, Erica
AU - Matusali, Giulia
AU - Cohen, Éric A.
AU - Doria, Margherita
PY - 2013/12
Y1 - 2013/12
N2 - Viral infection may induce the cell-surface expression of PVR (CD155) that, upon recognition by its cognate activating DNAM-1 receptor present on cytotoxic lymphocytes, may promote antiviral immune responses. Here we show that expression of the human immunodeficiency virus type 1 (HIV-1) Vpr protein in Jurkat T cells increases cell-surface and total PVR levels. Analysis of mutated Vpr variants indicated that Vpr uses the same protein surfaces, and hence probably the same mechanisms, to upregulate PVR and arrest the cell cycle in the G2 phase. Moreover, we found that PVR upregulation by Vpr relied on the ability of the protein to activate the ATR kinase that triggers the DNA damage response pathway and G2 arrest. Finally, we showed that Vpr contributes to PVR up-modulation in HIV-infected CD4+ T lymphocytes and inhibits the PVR downregulating activity of the viral Nef protein.
AB - Viral infection may induce the cell-surface expression of PVR (CD155) that, upon recognition by its cognate activating DNAM-1 receptor present on cytotoxic lymphocytes, may promote antiviral immune responses. Here we show that expression of the human immunodeficiency virus type 1 (HIV-1) Vpr protein in Jurkat T cells increases cell-surface and total PVR levels. Analysis of mutated Vpr variants indicated that Vpr uses the same protein surfaces, and hence probably the same mechanisms, to upregulate PVR and arrest the cell cycle in the G2 phase. Moreover, we found that PVR upregulation by Vpr relied on the ability of the protein to activate the ATR kinase that triggers the DNA damage response pathway and G2 arrest. Finally, we showed that Vpr contributes to PVR up-modulation in HIV-infected CD4+ T lymphocytes and inhibits the PVR downregulating activity of the viral Nef protein.
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UR - http://www.scopus.com/inward/citedby.url?scp=84887508280&partnerID=8YFLogxK
U2 - 10.1099/vir.0.055541-0
DO - 10.1099/vir.0.055541-0
M3 - Article
C2 - 24045107
AN - SCOPUS:84887508280
VL - 94
SP - 2664
EP - 2669
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - PART 12
ER -